FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2002089
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAGAGGCCAAGGTTGTAGTGGTGATAGCTGCTACCC82170.41042131493654876No Hit
GTAATATACAGCCGTGTCCTCGGCTCGCAGGCTGTTCATTTGCAGATACA79950.39933289678930356No Hit
GACTATTACTGTGCCGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGG74370.3714620079327143No Hit
GTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG72630.3627710856010896No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC71310.35617797210813307No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC59280.29609073322914214No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT55760.27850909724792455No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCATTCAGG54940.2744133752295727No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC49280.24614290373704667No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA48770.24359556443294977No Hit
ATAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCGAG47710.23830109450678766No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42850.21402644937362925No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT40590.20273823990841566No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC40490.2022387616134947No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC39140.19549580463206181No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA36690.18325858640649842No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36360.18161030803325928No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35020.17491729888131846No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT34630.17296933353112673No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC34270.1711712116694113No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGTCAAGCCTGGGG33420.16692564616258318No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA33030.16497768081239145No Hit
GATTATTATTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGG33000.16482783732391518No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32990.1647778894944231No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA32150.16058227181708704No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA31490.15728571507060876No Hit
GCTGTATATTACTGTGCGAGAGGCCAAGGTTGTAGTGGTGATAGCTGCTA31080.15523785406143284No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG30330.15149176684952567No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30310.15139187119054148No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA30200.15084244506612843No Hit
CTATTACTGTGCCGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCG30170.15069260157765213No Hit
GGTTACATATATTACGCAGACTCACTGAGGGGCCGATTCACCATCTCCAG30010.14989343630577862No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC29760.1486447405684762No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29450.14709635785422126No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAACCATTCAGGC29350.1465968795593003No Hit
ATATACAGCCGTGTCCTCGGCTCGCAGGCTGTTCATTTGCAGATACAGCG29220.14594755777590307No Hit
ATATTACGCAGACTCACTGAGGGGCCGATTCACCATCTCCAGAGACAACG29200.14584766211691888No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA29170.14569781862844258No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC28940.1445490185501244No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC27960.13965413125989903No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC27800.1388549659880255No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT27650.13810574854564406No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA26810.13391013086830805No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA26730.1335105482323713No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG26450.13211200900659262No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT25910.12941482621401945No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25880.12926498272554318No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA24560.12267186923258656No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24280.12127333000680789No Hit
ATATTACTGTGCGAGAGGCCAAGGTTGTAGTGGTGATAGCTGCTACCCAC23840.1190756255091557No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG23680.11827646023728215No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC23330.11652828620505881No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG23190.11582901659216947No Hit
TTACTGTGCCGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCGGAG23090.11532953829724853No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT22960.11468021651385128No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG22780.11378115558299355No Hit
CAGTAATAGTCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGA22700.1133815729470568No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG22230.11103402496092832No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG21820.1089861639517524No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21500.10738783340800534No Hit
GTCTGAGGATGAGGCTGACTATTACTGTGCCGCATGGGATGACAGCCTGA21310.10643882464765551No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21180.10578950286425828No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT21110.10543986805781362No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT20680.1032921113896535No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCGAGTCTGGCACCTCAG20580.10279263309473255No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCGAGTCTGGCACCTCAGCC20570.10274268526524045No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG20500.10239305045879579No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC20270.1012442503804776No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20140.10059492859708036No Hit
GTAATAATGTAAACTGGTACCAGCAACTCCCAGGAACGGCCCCCAAACTC20100.10039513727911197No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12300.090.151021
GTATCAA34350.072.318161
ATCAACG39000.063.692433
TCAACGC39750.062.490694
TATCAAC39850.062.153192
CAACGCA44600.055.695175
AACGCAG46700.052.8823286
CGCAGAG49100.049.710898
GCAGAGT54100.044.8458829
GTACATG47350.036.6481551
TATTGAG25750.036.069975
TACATGG47600.035.5462882
ACATGGG48400.034.8099823
GAATACG3750.034.5566149
ATTGAGG12450.034.1205446
TTATTGA28050.033.1123624
AATATAC42450.031.2085383
CAGCCGT41550.030.6684749
TATACAG44250.030.5898885
AAATCCG3850.029.9221365
Produced by FastQC (version 0.11.5)