FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000309
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG51380.5136412848429835No Hit
GTACAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT42820.42806772707233465No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC36960.36948582887887643No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCATTGGGTGTT35270.352591049365746No Hit
GATGTATACTGGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTCCTCAT32240.32230040917356534No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACCACTC32150.321400687187659No Hit
CTGTACAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGT27630.276214649673251No Hit
GTAATAGACGGCCGTGTCCTCGGATCTCAGGCTGCTGAGCTCCATGTAGG26250.2624189125560202No Hit
GTCCTACTACTATCATATGGACGTCTGGGGCCAAGGGACCACGGTCACCG25960.2595198083792108No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA25340.2533217235874115No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA22640.22633006401022085No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT22330.22323102161432118No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACGC22260.22253123784750514No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC20170.20163769395256867No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20070.20063800285711714No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACGCCTGGGCAGAGGGTCAT19710.19703911491349171No Hit
CCCAATGACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCACCC19630.19623936203713052No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA19560.19553957827031448No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19460.19453988717486298No Hit
GGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTCCTCATTAAAAGGAAT19460.19453988717486298No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACCACTCA18980.18974136991669574No Hit
ATACTGGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTCCTCATTAAAA18580.18574260553488972No Hit
GTCTATTACTGTGTGGCAGCGCCCCCCGGGGATTTTGGAGTGGTTATTGG17490.1748459725944683No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA17390.17384628149901682No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA17310.1730465286226556No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA17170.1716469610890235No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16980.16974754800766564No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16670.16664850561176597No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG16590.16584875273540475No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16440.1643492160922275No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG16000.15995057527224088No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC15780.15775125486224756No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15760.15755131664315727No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT15690.1568515328763412No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15630.1562517182190703No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT15510.15505208890452848No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA15500.15495211979498336No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15120.15115329363226762No Hit
GTATACTGGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTCCTCATTAA15100.1509533554131773No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15000.14995366431772583No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT14900.14895397322227433No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC14280.142755888430475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14110.14105641356820742No Hit
ATCCCATGCTGCACAGTAATAATCACCCTCATCCTCGGACCGGAGCCCAC14030.14025666069184622No Hit
GCCTACATGGAGCTCAGCAGCCTGAGATCCGAGGACACGGCCGTCTATTA14030.14025666069184622No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA13660.13655780363867565No Hit
CCTCACTCACTGTACAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC13570.1356580816527693No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13200.13195922459959875No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG13040.13035971884687633No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG12960.1295599659705151No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC12780.12776052199870241No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT12730.12726067645097663No Hit
TCCTCACCCTCCTCACTCACTGTACAGGGTCCTGGGCCCAGTCTGTGCTG12680.12676083090325088No Hit
GGTTATTGGTAGGTCCTACTACTATCATATGGACGTCTGGGGCCAAGGGA12560.1255612015887091No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC12550.12546123247916394No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC12550.12546123247916394No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCATTGGGTGTTCG12530.12526129426007362No Hit
TAGTAGGACCTACCAATAACCACTCCAAAATCCCCGGGGGGCGCTGCCAC12390.12386172672644152No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA12370.12366178850735123No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12080.12076268433054187No Hit
GCATGGGATGACAGCCTGAGTGGTCATTGGGTGTTCGGCGGAGGGACCAA11990.1198629623446355No Hit
GTCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC11850.1184633948110034No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA11620.11616410529146494No Hit
CACCAGGGACATGTCCACAAGCACAGCCTACATGGAGCTCAGCAGCCTGA11600.11596416707237464No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11540.11536435241510372No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG11510.11506444508646828No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11480.11476453775783282No Hit
GTATACATCATTACTTCCGATGTTGGAACTACCAGAACAAGAGATGATGA11420.11416472310056193No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11330.11326500111465557No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC11320.11316503200511042No Hit
CCATGTAGGCTGTGCTTGTGGACATGTCCCTGGTGAGGGTGACTCTTTCC11160.11156552625238801No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA11100.11096571159511712No Hit
CTCACTGTACAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCA11050.11046586604739135No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG11030.11026592782830105No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10980.10976608228057531No Hit
GTAATGATGTATACTGGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTC10980.10976608228057531No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10660.1065670707751305No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG10530.10526747235104353No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10510.10506753413195323No Hit
CAGTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA10370.10366796659832113No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCATTGGGTGTTCGGCG10310.1030681519410502No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10300.10296818283150506No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC10260.10256830639332447No Hit
GGATAGGATGGATCGTCGTTGGCAGTGGTTCCACAAAATTCGCTCACCAG10250.10246833728377931No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10190.1018685226265084No Hit
TCCTCACTCACTGTACAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCA10150.10146864618832781No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA10070.10066889331196661No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA21550.070.970431
ATCAACG22200.067.819023
TCAACGC22600.066.618684
CAACGCA23300.064.614035
TATCAAC23900.062.9950752
AACGCAG24150.062.2775086
CTACGCG350.003658089861.746363
ACGCAGA27250.055.192737
CGCAGAG27800.054.1007848
CGTATAG400.006206049654.0280723
GCAGAGT30750.048.910639
GAATACG4700.047.4641769
GTACATG26200.038.8245741
GTGCTAG5950.038.7991181
TGTACCG951.0687191E-437.912545
GAGTTTA4950.034.9779971
AAGACCG850.002830081433.898275
AATACGG850.002843992633.8643846
AGAATAC6750.033.049138
TGCTAGA7950.032.6207242
Produced by FastQC (version 0.11.5)