FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000309
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT49440.49424727759122433No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC35260.3524910802562008No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCATTGGGTGTT34660.34649293368349177No Hit
GATGTATACTGGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTCCTCAT33990.33979500334396673No Hit
CTGTACAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGT31010.31000420869951184No Hit
GTAATAATCACCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG28220.28211282713641483No Hit
GTCCTACTACTATCATATGGACGTCTGGGGCCAAGGGACCACGGTCACCG27050.2704164413196322No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACGC26410.2640184183087426No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACCACTC26260.26251888166556536No Hit
GTAATAGACGGCCGTGTCCTCGGATCTCAGGCTGCTGAGCTCCATGTAGG22840.22832944620112386No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACGCCTGGGCAGAGGGTCAT22010.22003201010887635No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT21670.21663306038434124No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA21480.2147336473029834No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC19930.19923843532348504No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA19590.19583948559894993No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA19400.19394007251759207No Hit
GGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTCCTCATTAAAAGGAAT18860.18854174060215395No Hit
TAGTAGGACCTACCAATAACCACTCCAAAATCCCCGGGGGGCGCTGCCAC18650.18644238930170576No Hit
ATACTGGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTCCTCATTAAAA18400.18394316156307702No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC18120.1811440264958128No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG17500.17494594170401345No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT17060.17054730088402684No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA16680.1667484747213111No Hit
GTCTATTACTGTGTGGCAGCGCCCCCCGGGGATTTTGGAGTGGTTATTGG16640.16634859828313053No Hit
GTCTGGGCCTGAGGTGAAGAAGCCTGGGACCTCAGTGAAGGTCTCCTGCA16520.16514896896858872No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA15970.1596506679436054No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15930.15925079150542482No Hit
GTTCCTCACTATGGACTGGATTTGGAGGATCCTCTTCTTGGTGGGAGCAG15450.1544522742472576No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15370.15365252137089638No Hit
GTATACTGGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTCCTCATTAA15320.15315267582317063No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA15270.15265283027544488No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAATGACCACTCA15200.15195304650862884No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT15060.1505534789749967No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT14940.14935384966045492No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14860.14855409678409373No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14810.14805425123636795No Hit
CCTCACTCACTGTACAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC14700.14695459103137132No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14450.14445536329274256No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA14030.14025666069184622No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13910.1390570313773044No Hit
TCCTCACCCTCCTCACTCACTGTACAGGGTCCTGGGCCCAGTCTGTGCTG13820.13815730939139806No Hit
ACCAATAACCACTCCAAAATCCCCGGGGGGCGCTGCCACACAGTAATAGA13800.13795737117230777No Hit
GCCTACATGGAGCTCAGCAGCCTGAGATCCGAGGACACGGCCGTCTATTA13240.13235910103777934No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT13210.13205919370914387No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13130.13125944083278268No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC13080.13075959528505693No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12930.12926005864187967No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA12800.1279604602177927No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC12740.12736064556052182No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC12700.1269607691223412No Hit
CCCAATGACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCACCC12660.1265608926841606No Hit
GGTTATTGGTAGGTCCTACTACTATCATATGGACGTCTGGGGCCAAGGGA12470.12466147960280274No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG12120.12116256076872246No Hit
GTCATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC12050.12046277700190641No Hit
CTCACTGTACAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCA11770.1176636419346422No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG11720.11716379638691644No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC11710.1170638272773713No Hit
TTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCATTGGGTGTTCG11630.11626407440101008No Hit
GCATGGGATGACAGCCTGAGTGGTCATTGGGTGTTCGGCGGAGGGACCAA11580.11576422885328434No Hit
GTAATGATGTATACTGGTATCAGCAGTTCCCAGGAACGGCCCCCAAACTC11510.11506444508646828No Hit
GTATACATCATTACTTCCGATGTTGGAACTACCAGAACAAGAGATGATGA11110.11106568070466227No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG11000.10996602049966561No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10920.10916626762330439No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA10900.1089663294042141No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG10850.10846648385648835No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG10820.1081665765278529No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10770.10766673098012713No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG10760.10756676187058198No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG10580.10576731789876928No Hit
CCATGTAGGCTGTGCTTGTGGACATGTCCCTGGTGAGGGTGACTCTTTCC10450.10446771947468232No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10200.10196849173605355No Hit
TCCTCACTCACTGTACAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCA10130.10126870796923751No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACGCCTGGGCAGAGG10120.10116873885969235No Hit
GTGCAGCATGGGATGACAGCCTGAGTGGTCATTGGGTGTTCGGCGGAGGG10040.10036898598333115No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCATTGGGTGTTCGGCG10030.10026901687378599No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)