FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737633
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT23990.32522948403881063No Hit
GTAATAAACGGCCGTGTCCTCAGATCTCAGGCTGCTCACCTCCATGTAGG17700.23995672644797614No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC15770.21379195345110646No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15050.204031001866782No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14230.19291436256241246No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14180.19223651870238995No Hit
GTTTATTACTGTGCGCGCGGGAAACGTGCATATAGTGCCTACGACCCACA13440.18220442957405647No Hit
GTCTGGGGCTGAGGTGGAGAAGCCTGGGTCCTCGGTGAGGGTCTCCTGCA13370.18125544817002492No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAA13290.1801708979939889No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12700.17217234044572302No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12420.16837641482959684No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA12140.16458048921347065No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11970.16227582008939406No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11930.16173354500137604No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC11850.16064899482533995No Hit
TTATTACTGTGCGCGCGGGAAACGTGCATATAGTGCCTACGACCCACAAC11540.15644636289320027No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11300.15319271236509213No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC11250.1525148685050696No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG11160.15129474955702904No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10620.14397403586878568No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA10160.1377378723565784No Hit
GCCTACATGGAGGTGAGCAGCCTGAGATCTGAGGACACGGCCGTTTATTA10060.13638218463653334No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG10060.13638218463653334No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9980.13529763446049728No Hit
GAGTTACAGTACCCCAAGCGCTTTCGGCGGAGGGACCAAGGTGGAGATCA9950.13489092814448378No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9860.13367080919644322No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCACCAAGGTTCAGTG9730.13190841516038462No Hit
TGCATATAGTGCCTACGACCCACAACCCGATGCTTTTGATATCTGGGGCC9460.12824805831626296No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9020.12228303234806469No Hit
CTATATGCACGTTTCCCGCGCGCACAGTAATAAACGGCCGTGTCCTCAGA8910.12079177585601511No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA8640.11713141901189345No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT8630.11699585023988894No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG8530.11564016251984388No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8420.11414890602779432No Hit
ACATGGAGGTGAGCAGCCTGAGATCTGAGGACACGGCCGTTTATTACTGT8250.1118442369037177No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8110.10994627409565462No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7980.10818388005959603No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7930.1075060361995735No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG7930.1075060361995735No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7910.10723489865556447No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7780.10547250461950591No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7740.1049302295314879No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAAGGATCATCCCTATTTTTGGT7690.10425238567146534No Hit
CATCATCGACAGTCTGCAACCTGAAGACTTTGCAACTTATTACTGTCAAC7640.10357454181144281No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT7600.10303226672342479No Hit
ACAGTACCCCAAGCGCTTTCGGCGGAGGGACCAAGGTGGAGATCAAACGA7570.10262556040741128No Hit
ATATGCACGTTTCCCGCGCGCACAGTAATAAACGGCCGTGTCCTCAGATC7540.10221885409139775No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT7410.10045646005533916No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7400.10032089128333467No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)