FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences737633
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21540.2920151348977066No Hit
GTAATAAACGGCCGTGTCCTCAGATCTCAGGCTGCTCACCTCCATGTAGG16990.2303313436356562No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15350.2080980650269172No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14170.19210094993038543No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13890.18830502431425927No Hit
GTCTGGGGCTGAGGTGGAGAAGCCTGGGTCCTCGGTGAGGGTCTCCTGCA13250.17962862290597084No Hit
GTTTATTACTGTGCGCGCGGGAAACGTGCATATAGTGCCTACGACCCACA12720.17244347798973203No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12440.16864755237360585No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12370.1676985709695743No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11770.15956444464930392No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGACCATTAGCAA11550.1565819316652048No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA11420.1548195376291462No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10870.14736325516889834No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC10540.14288948569274965No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA10320.13990697270865052No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10250.13895799130461897No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9800.1328573965644162No Hit
GAGTTACAGTACCCCAAGCGCTTTCGGCGGAGGGACCAAGGTGGAGATCA9560.12960374603630803No Hit
TTATTACTGTGCGCGCGGGAAACGTGCATATAGTGCCTACGACCCACAAC9530.1291970397202945No Hit
GCCTACATGGAGGTGAGCAGCCTGAGATCTGAGGACACGGCCGTTTATTA9490.12865476463227649No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG9450.12811248954425847No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9390.1272990769122314No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG9300.12607895796419086No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9280.12580782042018185No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9270.12567225164817733No Hit
CTATATGCACGTTTCCCGCGCGCACAGTAATAAACGGCCGTGTCCTCAGA9180.1244521327001368No Hit
GATCTATGCTGCATCCAGTTTGCAGAGTGGGGTCCCACCAAGGTTCAGTG9050.12268973866407822No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8930.12106291340002415No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8810.11943608813597005No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8540.11577573129184839No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8400.1138777684837853No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG8380.11360663093977628No Hit
TGCATATAGTGCCTACGACCCACAACCCGATGCTTTTGATATCTGGGGCC8370.11347106216777177No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG8210.11130196181569968No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8060.10926843023563208No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG8050.10913286146362758No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC7980.10818388005959603No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT7860.10655705479554196No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7740.1049302295314879No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7490.10154101023137523No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8750.074.818041
ATCAACG10100.064.079173
TATCAAC10500.061.638062
TCAACGC10850.059.6497424
CAACGCA11600.055.1656535
AACGCAG12650.051.1203236
CGCAGAG13400.047.797588
ACGCAGA16850.038.8653147
GAATACG2850.037.876269
GTACATG19600.035.6031651
GGGCAGT5500.035.3162041
GTGCTAG3000.033.5721971
ACATGGG20250.033.3809433
GGTGGGT5900.030.4935998
GACCGGT950.00490087730.3010087
AGTACCG950.004922114330.2742315
CATGGGG10750.028.095494
AGTTACC17450.028.0436259
TTACACG1306.7978445E-427.6581064
GGAGTTA17750.027.5696497
Produced by FastQC (version 0.11.5)