FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753803
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCA46910.6223111343414659No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT39450.5233462854353192No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC31780.42159556276639915No Hit
CTATAATATTGCTGACAGGAATAAACTGCCACATCTTCAGCCTGCAGGCT24240.32156942861729126No Hit
ATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA22150.29384335164492575No Hit
GTAATACATGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGGACG21910.29065949591604173No Hit
TTATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAC21620.28681233691030683No Hit
GTTTTGGACAGCTCCAAGAATAAGAATTCCATAGCTTGGTACCAGCAGAA20500.2719543435088478No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT20350.26996443367829526No Hit
ATGTATTACTGTGCGAGAGGCCCCGCGTATTACTATGATTCTACTGGTTA18580.24648349767777525No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG18360.2435649632596315No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT16600.22021668791448162No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15780.20933851417412772No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14990.19885832239988432No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG14780.19607244863711076No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14330.19010271914545313No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14110.18718418472730938No Hit
GCTCCAAGAATAAGAATTCCATAGCTTGGTACCAGCAGAAACCAGGACAG13840.18360234703231482No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13680.18147977654639208No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12720.16874435363085583No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12680.16821371100937513No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12430.1648971946251209No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12160.1613153569301263No Hit
GTACTATAATATTGCTGACAGGAATAAACTGCCACATCTTCAGCCTGCAG12100.1605193929979053No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA12030.1595907684103141No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11950.15852948316735274No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG11410.15136580777736358No Hit
AACCAGGACAGCCTCCTAAGATCCTCATTTACTGGGCATCTACCCGGGAA11370.1508351651558829No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11370.1508351651558829No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11290.14977387991292154No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA11080.14698800615014798No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA11050.14659002418403747No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10940.1451307569749656No Hit
CCTAAGATCCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA10900.14460011435348494No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCAC10630.14101827665849034No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10520.1395590094494185No Hit
TACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCT10230.13571185044368356No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT10210.13544652913294322No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC10130.13438524388998185No Hit
GTATTACTGTGCGAGAGGCCCCGCGTATTACTATGATTCTACTGGTTATT10110.13411992257924152No Hit
GTACATGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCA10020.13292597668091No Hit
GAGTACTATAATATTGCTGACAGGAATAAACTGCCACATCTTCAGCCTGC9960.132130012748689No Hit
GCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGATCCTCATTTACTG9800.13000744226276628No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG9760.12947679964128558No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9680.12841551439832424No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG9610.12748688981073306No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC9580.12708890784462254No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA9580.12708890784462254No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9570.12695624718925236No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTT9540.12655826522314184No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9500.12602762260166117No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG9300.12337440949425778No Hit
CTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCA9270.12297642752814728No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA9230.12244578490666659No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC9130.1211191783529649No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9090.12058853573148423No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG8700.11541477017204761No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8510.11289421772001439No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT8440.11196559313242319No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTCCTGTCAGC8370.111036968544832No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA8350.11077164723409168No Hit
GTTTATTCCTGTCAGCAATATTATAGTACTCCATTCACTTTCGGCCCTGG8310.110241004612611No Hit
TTCCATAGCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGATCCTCA8310.110241004612611No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8270.10971036199113032No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA8240.1093123800250198No Hit
GTATCAACGCAGAGTACATGGGGAGCTACAACAGGCAGGCAGGGGCAGCA7930.10519989970854454No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG7890.10466925708706386No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCC7860.10427127512095336No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7800.10347531118873235No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA7730.10254668660114115No Hit
CAGTAATACATGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGGA7690.10201604397966048No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7570.10042411611521844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)