FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753803
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCA48150.638761055607367No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT36390.4827521248920474No Hit
CTATAATATTGCTGACAGGAATAAACTGCCACATCTTCAGCCTGCAGGCT32070.4254427217721341No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC24740.3282024613857998No Hit
GTAATACATGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGGACG21100.279913982831058No Hit
ATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA20990.27845471562198615No Hit
TTATAGTACTCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAC19990.26518865008496917No Hit
GTTTTGGACAGCTCCAAGAATAAGAATTCCATAGCTTGGTACCAGCAGAA19160.2541778156892451No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17960.23825853704482472No Hit
ATGTATTACTGTGCGAGAGGCCCCGCGTATTACTATGATTCTACTGGTTA17230.2285743092028023No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT15340.20350144533784026No Hit
GTACTATAATATTGCTGACAGGAATAAACTGCCACATCTTCAGCCTGCAG14780.19607244863711076No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14500.19235795028674602No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14190.18824546997027075No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13550.1797551880265799No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13390.17763261754065718No Hit
GAGTACTATAATATTGCTGACAGGAATAAACTGCCACATCTTCAGCCTGC12920.1713975667382592No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12920.1713975667382592No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12890.1709995847721487No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC12690.1683463716647453No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12160.1613153569301263No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA11870.1574681979243914No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT11530.15295773564180562No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11120.14751864877162868No Hit
GCTCCAAGAATAAGAATTCCATAGCTTGGTACCAGCAGAAACCAGGACAG11080.14698800615014798No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11070.1468553454947778No Hit
GTCCCTGACCGATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCAC10940.1451307569749656No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10800.14327350779978323No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATG10670.141548919279971No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA10520.1395590094494185No Hit
GTCCCAGACCCGCTGCCACTGAATCGGTCAGGGACCCCGGATTCCCGGGT10360.13743643896349578No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10350.1373037783081256No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG10100.13398726192387134No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA10080.13372194061313103No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9990.1325279947147995No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9940.13186469143794863No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9820.1302727635735066No Hit
GACTTCACTCTCACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTA9810.13014010291813644No Hit
CCTAAGATCCTCATTTACTGGGCATCTACCCGGGAATCCGGGGTCCCTGA9490.12589496194629102No Hit
AACCAGGACAGCCTCCTAAGATCCTCATTTACTGGGCATCTACCCGGGAA9480.12576230129092084No Hit
GTATTACTGTGCGAGAGGCCCCGCGTATTACTATGATTCTACTGGTTATT9480.12576230129092084No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC9360.12417037342647881No Hit
GTGCAGCCACAGTTCGTTTGATATCCACTTTGGTCCCAGGGCCGAAAGTG9240.12257844556203676No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC9180.12178248162981575No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC9130.1211191783529649No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA9120.12098651769759472No Hit
ATTCAGTGGCAGCGGGTCTGGGACAGACTTCACTCTCACCATCAGCAGCC9010.11952725048852288No Hit
GTGTTGCAGACCCAGGTCTTCATTTCTCTGTTGCTCTGGATCTCTGGTGC8880.11780266196871066No Hit
GTTTATTCCTGTCAGCAATATTATAGTACTCCATTCACTTTCGGCCCTGG8710.11554743082741778No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8530.11315953903075471No Hit
GCTTGGTACCAGCAGAAACCAGGACAGCCTCCTAAGATCCTCATTTACTG8470.1123635750985337No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG8340.11063898657872148No Hit
GTACATGGGGAGCTACAACAGGCAGGCAGGGGCAGCAAGATGGTGTTGCA8300.11010834395724081No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG8260.10957770133576013No Hit
GTCTCCAGACTCCCTGGCTGTGTCTCTGGGCGAGAGGGCCACCATCAACT8230.10917971936964964No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8080.10718980953909708No Hit
GTCTCTGGGCGAGAGGGCCACCATCAACTGCAAGTCCAGCCAGAGTGTTT7930.10519989970854454No Hit
TACGGGGACATCGTGATGACCCAGTCTCCAGACTCCCTGGCTGTGTCTCT7910.10493457839780419No Hit
CAGTAATACATGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGGA7880.1045365964316937No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG7810.1036079718441025No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA7730.10254668660114115No Hit
CACCATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTCCTGTCAGC7650.1014854013581798No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA7560.10029145545984826No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)