FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1436031
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT90320.6289557815952441No Hit
ACATTACCCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAGTCAAACGA72560.5052815712195629No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62360.4342524639092053No Hit
GGGTAATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGC52430.3651035388511808No Hit
GTCCTGATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT51180.3563989913866762No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT47580.33132989468890295No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGCAG45270.31524389097449845No Hit
GATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCATTG44420.30932479869863533No Hit
GAGTTACATTACCCCGTGGACGTTCGGCCCAGGGACCAAGGTGGAAGTCA43210.30089879675299486No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43130.3003417057152666No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT37450.2607882420365577No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG36570.25466024062154646No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG33620.23411750860531563No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33160.23091423513837794No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG32840.22868587098746473No Hit
ATGTAACTCTGTTGACAGTAGTAAGTTGCAAAATCCTCAGGTTGCAGACT31860.22186150577529315No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31190.2171958683343187No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29670.20661113861748112No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT28710.19992604616474158No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG28280.19693168183695198No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA27530.19170895335824922No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA26610.18530240642437384No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA23590.16427221975013076No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG23580.1642025833704147No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23410.1630187649152421No Hit
ATGCTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCATTGGCAGT23160.16127785542234116No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA22950.15981549144830437No Hit
ACTTACTACTGTCAACAGAGTTACATTACCCCGTGGACGTTCGGCCCAGG22440.15626403608278652No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA22080.15375712641300918No Hit
GATCAGGACCTTAGGGGCTTTCTCTGGTTTCTGCTGATACCAATTTAAAT22000.1532000353752809No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21910.15257330795783655No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTGGGCCGAACGTC21800.15180730778096016No Hit
CACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAAC21350.14867367069373852No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC21090.14686312482112154No Hit
GTCTGCAACCTGAGGATTTTGCAACTTACTACTGTCAACAGAGTTACATT21060.14665421568197345No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATTTTGCAACTTA20470.14254566927872728No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC20340.1416403963424188No Hit
GTCTCCGTCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT19900.13857639563491317No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19620.13662657700286415No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC19470.1355820313071236No Hit
GTAGTAAGTTGCAAAATCCTCAGGTTGCAGACTGCTGATGGTGAGAGTGA19460.13551239492740755No Hit
ATTTAAATTGGTATCAGCAGAAACCAGAGAAAGCCCCTAAGGTCCTGATC19190.13363221267507455No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT18530.12903621161381612No Hit
CCCCTAAGGTCCTGATCTATGCTGCATCCAGTTTGGAAAGTGGGGTCCCA17710.12332602847710111No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA17620.12269930105965679No Hit
ATCTGGGACAGATTTCACTCTCACCATCAGCAGTCTGCAACCTGAGGATT17560.12228148278136057No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17530.12207257364221247No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG17210.11984420949129929No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCC17160.1194960275927191No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT17060.11879966379555872No Hit
GGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCC16770.11678020878379367No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT16430.11441257187344842No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC16410.11427329911401635No Hit
CTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGC16400.1142036627343003No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16290.1134376625574239No Hit
CTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCCGGATGACCCAGTC16060.11183602582395505No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16040.11169675306452298No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG15770.10981657081218998No Hit
GCATAGATCAGGACCTTAGGGGCTTTCTCTGGTTTCTGCTGATACCAATT15660.10905057063531358No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAG15660.10905057063531358No Hit
CCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTG15570.10842384321786926No Hit
ACTGTCAACAGAGTTACATTACCCCGTGGACGTTCGGCCCAGGGACCAAG15420.1073792975221287No Hit
GACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGGCTCCG15110.10522056975093155No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCT15080.10501166061178345No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA15060.10487238785235138No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC14790.10299220560001839No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA14560.10139056886654954No Hit
GCTCCTGGGGCTCCTGCTACTCTGGCTCCGAGGTGCCAGATGTGACATCC14460.10069420506938916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)