FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331406
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC5400.16294213140377664No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA5190.15660549296029644No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT5010.15117408858017056No Hit
ATAGTACACGGCCGTGTCCTCAGATCTCAGGCTGCTCATCTCCATGTAGG4790.1445357054489056No Hit
GCCTACATGGAGATGAGCAGCCTGAGATCTGAGGACACGGCCGTGTACTA4370.13186242856194516No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA4190.12643102418181926No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC4170.12582753480624972No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT4160.12552579011846496No Hit
GTCCGGGGCTGAGGTGAGGAAGCCTGGCTCGTCGGTGAAGGTCTCCTGCC4150.1252240454306802No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC4100.12371532199175633No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT3910.11798217292384566No Hit
GACGTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCGGATACCACTG3840.11586996010935229No Hit
CTCTTTGCATAGGGTCTGTGGTTTCTTCTGAGCTGACTCAGGACCCTGAT3840.11586996010935229No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT3810.11496472604599797No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA3800.11466298135821319No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT3740.11285251323150457No Hit
GGCCTGGACCCCTCTCTGGCTCACTCTCCTCACTCTTTGCATAGGGTCTG3620.10923157697808732No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG3600.10862808760251776No Hit
GTTCTGGGGTGTCTCCACCATGGCCTGGACCCCTCTCTGGCTCACTCTCC3460.10440366197353096No Hit
AGCCAGGACAGGCCCCTGTCCTTGTCGTCTATGGTACAAACAACCGGCCC3390.10229144915903755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG3200.074.2562263
TCAACGC3250.073.113824
GTATCAA3800.066.321351
CAACGCA3650.065.101345
AACGCAG4200.058.2818036
TATCAAC4400.057.2775272
CGCAGAG4350.054.617038
GGTATCA1402.910383E-1046.2895931
ACGCAGA5300.046.1855857
TCTACGT700.001091724741.1463052
CTAAGGT750.001532583138.403224
TACGTCT750.001532583138.403224
GTGCTAG1355.230813E-737.3364641
CTAGAGA1551.5412261E-632.5188564
CTACGTC5300.031.2479023
GTACATG7150.031.2194021
AGTTACC1851.7415186E-731.1330479
CTAGCAC950.004883367630.3183333
GGAGTTA1952.76239E-729.5364827
GTCTACG1255.3565827E-428.8024141
Produced by FastQC (version 0.11.5)