FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201996
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCACGTATCGCCAGTACAATGTTGGCCCTCCACGCCTTTCGCACA56880.4732128892275848No Hit
GTGCTACACTGGTCCATTCTACTACTACGGTATGCATGTCTGGGGCCAAG47260.39317934502277874No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCGGCTGGTGGAGTCTGGGGGAGGCG33980.2826964482410923No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC33450.27828711576411236No Hit
ATTTATTATTGTGCGAAAGGCGTGGAGGGCCAACATTGTACTGGCGATAC32980.2743769530015075No Hit
ATAATAAATAGCCGTGTCCTCAACTCTCAGGCTGTTCATTTGGAGATAGA32400.2695516457625483No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT31270.26015061614181745No Hit
CTCCAGAGACGATTCCAAGAACACCCTCTATCTCCAAATGAACAGCCTGA30240.2515815360450451No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29980.24941846728275302No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA29460.24509232975816891No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCATTGAGG28710.23885270832848027No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCATTGAGGC28500.2371056143281675No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC27350.22753819480264495No Hit
ATATTATGTAGACTCCGTGAAGGGCCGATTCACCCTCTCCAGAGACGATT27230.22653985537389476No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA25730.21406061251451752No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25400.21131517908545452No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC24380.20282929394107801No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT24310.20224692927430704No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC24160.20099900498836937No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA23990.19958469079763994No Hit
TCTCTGGAGAGGGTGAATCGGCCCTTCACGGAGTCTACATAATATTTAGT23170.19276270470118037No Hit
CATCTATAGTAATCATTATCGGCCCTCAGGGGTCCCTGACCGATTCTCTG22530.18743822774784608No Hit
ACATAATATTTAGTACTTCCATCATATGATATGACTGCCACCCACTCCAG22280.1853583539379499No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21210.1764564940315941No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC21140.17587412936482316No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21030.17495898488846884No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20950.17429342526930205No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20090.1671386593632591No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA19870.16530837041055044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19420.16156459755273728No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19190.15965111364763276No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT18770.15615692564700714No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC17920.14908535469336004No Hit
CTATAGATGAGGAGTTTGGGGGCCGCTCCTGGGAGTTGCTGGTACCAGTT17850.1485029900265891No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17770.1478374304074223No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGATTTTGACATGCACTGGG17480.14542477678794272No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAA17420.14492560707356764No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17370.1445096323115884No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC17200.143095318120859No Hit
ATCCCATGCTGCACAGTAAAAATCAGCCTCATCCTCAGACTGGAGCCCAC17190.14301212316846312No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16880.14043307964419183No Hit
TTCTACTACTACGGTATGCATGTCTGGGGCCAAGGGACCACGGTCACCGT16740.13926835031064994No Hit
GCATGGGATGACAGCCTCAATGGTTTTGTCTTCGGAACTGGGACCACGGT16740.13926835031064994No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT16690.1388523755486707No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG16240.13510860269085753No Hit
GTAAAAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG16200.13477582288127415No Hit
GTACAATGTTGGCCCTCCACGCCTTTCGCACAATAATAAATAGCCGTGTC16050.13352789859533643No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG15950.13269594907137794No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTT15610.1298673206899191No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA15430.12836981154679383No Hit
GGGCTGGAGTGGGTGGCAGTCATATCATATGATGGAAGTACTAAATATTA15190.12637313268929348No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA15170.12620674278450178No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC14950.1243764538317931No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14940.12429325887939728No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14730.12254616487908446No Hit
GTCCAGTGTCAGGTGCGGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC14650.12188060525991767No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT14650.12188060525991767No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA14590.12138143554554258No Hit
GTGCAGCATGGGATGACAGCCTCAATGGTTTTGTCTTCGGAACTGGGACC14500.12063268097397994No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14470.1203830961167924No Hit
GTAGTAGAATGGACCAGTGTAGCACGTATCGCCAGTACAATGTTGGCCCT14370.11955114659283392No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT14270.11871919706887545No Hit
GTCTACATAATATTTAGTACTTCCATCATATGATATGACTGCCACCCACT14260.11863600211647958No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC14090.11722168792575016No Hit
ACTGTAAACTGGTACCAGCAACTCCCAGGAGCGGCCCCCAAACTCCTCAT14090.11722168792575016No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13820.11497542421106226No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13770.11455944944908303No Hit
GTCTGAGGATGAGGCTGATTTTTACTGTGCAGCATGGGATGACAGCCTCA13740.11430986459189547No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC13720.11414347468710379No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGATTTTGAC13690.11389388982991624No Hit
TATGTAGACTCCGTGAAGGGCCGATTCACCCTCTCCAGAGACGATTCCAA13660.11364430497272869No Hit
ATAGTAATCATTATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCC13570.11289555040116606No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC13570.11289555040116606No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC13530.11256277059158266No Hit
ACATTGTACTGGCGATACGTGCTACACTGGTCCATTCTACTACTACGGTA13490.11222999078199927No Hit
GGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCCGGGGAGCGGGG13470.11206360087720758No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT13310.110732481638874No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA13200.10981733716251968No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC13150.10940136240054045No Hit
CTCCAGCCCCTTGCCTGGAGTCTGGCGGACCCAGTGCATGTCAAAATCAC13080.10881899773376949No Hit
GCCTGAGAGTTGAGGACACGGCTATTTATTATTGTGCGAAAGGCGTGGAG13040.1084862179241861No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12990.10807024316220686No Hit
TTATTATTGTGCGAAAGGCGTGGAGGGCCAACATTGTACTGGCGATACGT12850.10690551382866499No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12720.10582397944751895No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACGCAGCCACCCTCAGCGTCT12570.10457605516158125No Hit
ATGCTGCACAGTAAAAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG12500.1039936904948103No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG12420.10332813087564352No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT12410.10324493592324765No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG12400.1031617409708518No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG12270.10208020658970579No Hit
GATTTTTACTGTGCAGCATGGGATGACAGCCTCAATGGTTTTGTCTTCGG12090.10058269744658052No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC12090.10058269744658052No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12020.10000033277980959No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)