FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201996
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAGCACGTATCGCCAGTACAATGTTGGCCCTCCACGCCTTTCGCACA49580.41248057397861554No Hit
GTGCTACACTGGTCCATTCTACTACTACGGTATGCATGTCTGGGGCCAAG41150.34234722910891546No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCGGCTGGTGGAGTCTGGGGGAGGCG29780.24775456823483605No Hit
ATAATAAATAGCCGTGTCCTCAACTCTCAGGCTGTTCATTTGGAGATAGA29740.24742178842525267No Hit
ATTTATTATTGTGCGAAAGGCGTGGAGGGCCAACATTGTACTGGCGATAC29220.24309565090066854No Hit
GTCCTGGGCCCAGTCTGTCCTGACGCAGCCACCCTCAGCGTCTGGGACCC28720.23893590328087616No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACGCAGCCACCCTCAGCGTCT28070.233528231375146No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT27510.2288693140409785No Hit
CTCCAGAGACGATTCCAAGAACACCCTCTATCTCCAAATGAACAGCCTGA27200.22629027051670722No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC27180.2261238806119155No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT26320.21896911470587255No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25930.21572451156243447No Hit
ATATTATGTAGACTCCGTGAAGGGCCGATTCACCCTCTCCAGAGACGATT24220.20149817470274445No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC23820.19817037660691048No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA22020.18319528517565783No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT21990.18294570031847027No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC21940.18252972555649105No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21130.17579093441242732No Hit
CATCTATAGTAATCATTATCGGCCCTCAGGGGTCCCTGACCGATTCTCTG20690.17213035650701No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20180.16788741393482176No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCATTGAGG20080.16705546441086327No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20080.16705546441086327No Hit
GTCCTGACGCAGCCACCCTCAGCGTCTGGGACCCCCGGACAGAGGGTCAC20020.16655629469648817No Hit
TCTCTGGAGAGGGTGAATCGGCCCTTCACGGAGTCTACATAATATTTAGT20000.1663899047916965No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCATTGAGGC19770.16447642088659198No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACGCAGCCAC19270.16031667326679955No Hit
ACATAATATTTAGTACTTCCATCATATGATATGACTGCCACCCACTCCAG19170.1594847237428411No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA18940.15757123983773655No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG18210.15149800831283966No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA18070.15033327897929777No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA17470.14534158183554688No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT17380.14459282726398423No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17240.14342809793044237No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC17180.14292892821606726No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17020.14159780897773372No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC16530.13752125631033715No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG15620.12995051564231494No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG15600.12978412573752326No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT15400.1281202266896063No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGTGATTTTGACATGCACTGGG15310.12737147211804364No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC15010.1248756235461682No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14880.12379408916502219No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT14810.12321172449825124No Hit
TTCTACTACTACGGTATGCATGTCTGGGGCCAAGGGACCACGGTCACCGT14760.122795749736272No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG14660.12196380021231351No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT14550.12104865573595919No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14200.1181368324021045No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC14200.1181368324021045No Hit
ATAGTAATCATTATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCC14150.11772085764012526No Hit
GCATGGGATGACAGCCTCAATGGTTTTGTCTTCGGAACTGGGACCACGGT14110.11738807783054185No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14020.11663932325897922No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA13930.11589056868741661No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTT13930.11589056868741661No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13930.11589056868741661No Hit
GTACAATGTTGGCCCTCCACGCCTTTCGCACAATAATAAATAGCCGTGTC13870.11539139897304151No Hit
GGGCTGGAGTGGGTGGCAGTCATATCATATGATGGAAGTACTAAATATTA13760.11447625449668716No Hit
ACTGTAAACTGGTACCAGCAACTCCCAGGAGCGGCCCCCAAACTCCTCAT13690.11389388982991624No Hit
GTGCAGCATGGGATGACAGCCTCAATGGTTTTGTCTTCGGAACTGGGACC13380.11131484630564495No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT13240.11015011697210306No Hit
TATGTAGACTCCGTGAAGGGCCGATTCACCCTCTCCAGAGACGATTCCAA13100.10898538763856119No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC12940.10765426840022763No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12780.10632314916189405No Hit
GTCCAGTGTCAGGTGCGGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC12710.10574078449512311No Hit
GTCTACATAATATTTAGTACTTCCATCATATGATATGACTGCCACCCACT12680.10549119963793557No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT12680.10549119963793557No Hit
GTAGTAGAATGGACCAGTGTAGCACGTATCGCCAGTACAATGTTGGCCCT12470.10374410563762275No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12450.10357771573283106No Hit
CTATAGATGAGGAGTTTGGGGGCCGCTCCTGGGAGTTGCTGGTACCAGTT12350.10274576620887257No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12270.10208020658970579No Hit
GATTTTTACTGTGCAGCATGGGATGACAGCCTCAATGGTTTTGTCTTCGG12250.1019138166849141No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGTGATTTTGAC12160.10116506211335147No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT12160.10116506211335147No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAA12150.1010818671609556No Hit
ACATTGTACTGGCGATACGTGCTACACTGGTCCATTCTACTACTACGGTA12140.10099867220855975No Hit
GTCTGAGGATGAGGCTGATTTTTACTGTGCAGCATGGGATGACAGCCTCA12080.10049950249418468No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)