FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610262
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG22650.37115206255673794No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21340.34968587262520034No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTGTAGTCTTCGG19430.31838783997692793No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACTACACCACTCAGG16820.27561932415913165No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC14210.23285080834133534No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT14090.2308844397979884No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACTAC11710.1918847970216071No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11550.1892629722971445No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTACCGCCTGG10990.1800865857615254No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC10970.17975885767096755No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA10060.16484722955058648No Hit
GTGTAGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAG10010.16402790932419192No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9920.1625531329166817No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9740.15960358010166126No Hit
CTATAGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCT9720.15927585201110342No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG9600.15730948346775647No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.15714561942247754No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC9400.1540322025621782No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9370.15354061042634148No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9290.15222969806411019No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9270.15190196997355235No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC9040.14813309693213736No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT8830.14469195198128018No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8790.14403649580016453No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8630.1414146710757019No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT8630.1414146710757019No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8620.141250807030423No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8470.1387928463512393No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACTACACCACTCAGGC8420.13797352612484473No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT8320.13633488567205562No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8250.13518783735510323No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8200.13436851712870865No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7940.13010805195145692No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA7920.12978032386089908No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7770.1273223631817154No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7660.12551985868364735No Hit
AGACTACACCACTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC7590.12437281036669495No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC7430.12175098564223236No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7420.12158712159695345No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7410.12142325755167453No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA7290.11945688900832757No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT7200.11798211260081734No Hit
GTAATACACGGCTGTGTCCTCGGTTCTCAGGCTGTTCAGTTGCAAAAACA7180.11765438451025953No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC6890.11290232719717105No Hit
TTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA6800.11142755078966084No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG6750.11060823056326625No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG6660.10913345415575605No Hit
CATCTATAGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG6660.10913345415575605No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG6640.10880572606519823No Hit
GCATGGGATGACAGCCTGAGTGGTGTAGTCTTCGGCGGAGGGACCAAGTT6610.10831413392936148No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT6500.10651162943129346No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG6440.10552844515961998No Hit
GTACATGGGAGCTTCAGCTGAGGTAGAGAAGACAGGATTCAGGACAATCT6410.10503685302378322No Hit
GTGTATTACTGTACCACAGGGGGCCCCATATCATATTTGGAGTGGCTACC6240.1022511642540417No Hit
TATTTGGAGTGGCTACCTGTTGACTACTGGGGCCAGGGAACCCTGGTCAC6130.10044865975597367No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)