FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610262
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC22020.3608286277041664No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTGTAGTCTTCGG18620.305114852309336No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC17930.293808233185091No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT17290.28332093428724053No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACTACACCACTCAGG13530.22170805326236928No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC11120.18221681835015124No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10760.17631771272011038No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10260.16812451045616475No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10250.16796064641088582No Hit
CTATAGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCT10190.16697746213921236No Hit
GTGTAGTCTTCGGCGGAGGGACCAAGTTGACCGTCCTAGGTCAGCCCAAG9800.16058676437333474No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACTAC9790.1604229003280558No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG9760.15993130819221907No Hit
CCCTTAGACTCTCCTGTGCAGCCTCTGGATTCACTTTCAGTACCGCCTGG9750.15976744414694016No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC9700.1589481239205456No Hit
GTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG9620.1576372115583143No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9350.15321288233578365No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9210.15091878570187886No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9070.14862468906797408No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8740.14321717557376995No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT8400.13764579803428692No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8210.13453238117398758No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8130.13322146881175626No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7880.12912486767978343No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA7640.12519213059308953No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA7560.12388121823085822No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7510.12306189800446365No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7500.12289803395918475No Hit
GTACATGGGAGCTTCAGCTGAGGTAGAGAAGACAGGATTCAGGACAATCT7390.1210955294611167No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7290.11945688900832757No Hit
CATCTATAGTAATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG7270.11912916091776973No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT7230.1184737047366541No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7220.11830984069137519No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7060.11568801596691257No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT6920.11339391933300778No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT6870.11257459910661322No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA6840.11208300697077649No Hit
ACCTAGGACGGTCAACTTGGTCCCTCCGCCGAAGACTACACCACTCAGGC6770.11093595865382411No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC6670.10929731820103496No Hit
TTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCA6660.10913345415575605No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT6600.10815026988408258No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC6490.10634776538601452No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT6420.10520071706906213No Hit
GTAATACACGGCTGTGTCCTCGGTTCTCAGGCTGTTCAGTTGCAAAAACA6260.10257889234459953No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA6240.1022511642540417No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG6230.10208730020876279No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA6160.1009402518918104No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)