FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769250
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGTACATAGCCGTATCTCCCGGTCTCAGGCTGTTCATTTGCAAAAACA26740.34761130971725707No Hit
ATGTACTATTGTGCGAGAGAGGGAGTCTGTAGTGGTGCAACCTGTTACTC24910.3238219044523887No Hit
ATATTATGCAGACTCCGTGACGGACCGATTTACCATCTCCAGAGACAACA22620.2940526486837829No Hit
TTTAAGAGCTGTCCAGTGTCAGGTCCACCTGGTGGAGTCTGGGGGAAGCG22460.29197270068248293No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21180.2753331166720832No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21120.2745531361715957No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20180.2623334416639584No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19970.2596035099122522No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT19660.2555736106597335No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19660.2555736106597335No Hit
GTACTATTGTGCGAGAGAGGGAGTCTGTAGTGGTGCAACCTGTTACTCGT18840.24491387715307117No Hit
CAATAGTACATAGCCGTATCTCCCGGTCTCAGGCTGTTCATTTGCAAAAA18130.23568410789730257No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA17820.2316542086447839No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC17390.2260643483912902No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCGCCCTCAGG16950.22034449138771528No Hit
GTCCAGTGTCAGGTCCACCTGGTGGAGTCTGGGGGAAGCGTGGTCCAGCC16670.21670458238544038No Hit
CTCCTGCACTGGGAGCAAGTCCGAGATCGGGCCAGGTTATGATGTACACT15910.2068248293792655No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG14660.19057523561910952No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT14660.19057523561910952No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTCAGTAACTTTGCTATGCACTGGG14490.18836529086772832No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14480.18823529411764706No Hit
GCCTGAGACCGGGAGATACGGCTATGTACTATTGTGCGAGAGAGGGAGTC14090.18316542086447837No Hit
GCATAATATGTCTCACTTCCATCAGACGATATAACTGCCACCCACTCCAG14030.1823854403639909No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13530.17588560285992852No Hit
GTCTCAGGCTGTTCATTTGCAAAAACACCTTGTTTTTGGTGTTGTCTCTG13260.1723756906077348No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13230.17198570035749106No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC12890.16756581085472863No Hit
GGGCTGGAGTGGGTGGCAGTTATATCGTCTGATGGAAGTGAGACATATTA12530.1628859278518037No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG12460.16197595060123499No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT12280.1596360090997725No Hit
TGGTAGCACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCA12200.1585960350991225No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA11800.15339616509587262No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11490.1493662658433539No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11430.14858628534286641No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAGCACCAATCGGCCCT11340.1474163145921352No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11330.14728631784205395No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT11100.14429639259018523No Hit
TCCTATGACAGCAGCCTGAGGGCGGTGGTATTCGGCGGAGGGACCAAGCT10940.14221644458888527No Hit
GTCTCTGGATTCACCTTCAGTAACTTTGCTATGCACTGGGTCCGCCAGGC10800.1403964900877478No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA10800.1403964900877478No Hit
CTCCAGAGACAACACCAAAAACAAGGTGTTTTTGCAAATGAACAGCCTGA10800.1403964900877478No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG10690.13896652583685407No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10560.13727656808579786No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT10440.13571660708482286No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10420.13545661358466038No Hit
CTCCGTGACGGACCGATTTACCATCTCCAGAGACAACACCAAAAACAAGG10330.13428664283392916No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG10270.13350666233344166No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10150.1319467013324667No Hit
CCCTGAGACTCTCCTGTGCAGTCTCTGGATTCACCTTCAGTAACTTTGCT10120.13155671108222294No Hit
GTACATAGCCGTATCTCCCGGTCTCAGGCTGTTCATTTGCAAAAACACCT10120.13155671108222294No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG10080.13103672408189795No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC9990.12986675333116673No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT9930.12908677283067924No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC9910.12882677933051673No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9910.12882677933051673No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC9890.12856678583035425No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG9860.1281767955801105No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9830.12778680532986675No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG9770.12700682482937928No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCGCCCTCAGGC9690.12596685082872927No Hit
CTACAGACTCCCTCTCTCGCACAATAGTACATAGCCGTATCTCCCGGTCT9670.1257068573285668No Hit
ACTACAGACTCCCTCTCTCGCACAATAGTACATAGCCGTATCTCCCGGTC9580.12453688657783557No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC9550.12414689632759182No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT9380.12193695157621058No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9290.12076698082547936No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT9240.12011699707507313No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9210.11972700682482938No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9080.11803704907377315No Hit
GCTATGTACTATTGTGCGAGAGAGGGAGTCTGTAGTGGTGCAACCTGTTA9020.11725706857328567No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT8820.11465713357166071No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8820.11465713357166071No Hit
GTCTGTAGTGGTGCAACCTGTTACTCGTCATCTTTTGACTATTGGGGCCA8810.11452713682157947No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8790.11426714332141696No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC8730.11348716282092947No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8640.11231719207019823No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8620.11205719857003574No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC8610.11192720181995451No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8460.10997725056873578No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA8450.10984725381865454No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG8430.10958726031849203No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA8380.1089372765680858No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG8360.1086772830679233No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC8360.1086772830679233No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG8310.10802729931751705No Hit
GTGTCAGGTCCACCTGGTGGAGTCTGGGGGAAGCGTGGTCCAGCCTGGGA8250.10724731881702958No Hit
ATATAACTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAG8200.10659733506662332No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA8180.10633734156646085No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT8120.10555736106597335No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8070.10490737731556712No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC7950.10334741631459214No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA7940.10321741956451089No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT7930.10308742281442965No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA7910.10282742931426714No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC7890.10256743581410464No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA7860.10217744556386091No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA7840.10191745206369841No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT7800.10139746506337341No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4250.089.405091
GTATCAA11700.078.4331051
ATCAACG15100.061.111963
TCAACGC15300.060.277824
TATCAAC15750.058.6509442
CAACGCA15850.057.73515
AACGCAG16600.055.1158376
CGCAGAG18350.049.859568
CAGCGCT5550.045.0647169
GCAGAGT21300.042.9429559
ACGCAGA22350.041.2559627
GCAGCGC6250.040.0278748
GGCAGCG6800.036.790337
TATTGAG6750.036.0109375
TACATGG18500.035.6108172
GTACATG18800.035.465041
ACATGGG18850.034.9132233
CAGGGCA6700.034.1455734
GTACACG10450.032.8385665
GTATGTA11900.032.532851
Produced by FastQC (version 0.11.5)