FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769250
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGTACATAGCCGTATCTCCCGGTCTCAGGCTGTTCATTTGCAAAAACA23710.30822229444263893No Hit
ATGTACTATTGTGCGAGAGAGGGAGTCTGTAGTGGTGCAACCTGTTACTC23100.3002924926876828No Hit
ATATTATGCAGACTCCGTGACGGACCGATTTACCATCTCCAGAGACAACA21820.28365290867728304No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT21440.27871303217419563No Hit
TTTAAGAGCTGTCCAGTGTCAGGTCCACCTGGTGGAGTCTGGGGGAAGCG20860.2711732206694833No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18470.240103997400065No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18320.23815404614884625No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT18030.23438414039649008No Hit
GTACTATTGTGCGAGAGAGGGAGTCTGTAGTGGTGCAACCTGTTACTCGT17900.23269418264543384No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17760.2308742281442964No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC17510.22762430939226522No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17120.22255443613909653No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC16800.21839454013649662No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16350.2125446863828404No Hit
GTCCAGTGTCAGGTCCACCTGGTGGAGTCTGGGGGAAGCGTGGTCCAGCC15950.2073448163795905No Hit
CAATAGTACATAGCCGTATCTCCCGGTCTCAGGCTGTTCATTTGCAAAAA15340.19941501462463437No Hit
CTCCTGCACTGGGAGCAAGTCCGAGATCGGGCCAGGTTATGATGTACACT14950.19434514137146572No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCGCCCTCAGG14340.18641533961650958No Hit
GCATAATATGTCTCACTTCCATCAGACGATATAACTGCCACCCACTCCAG13610.1769255768605785No Hit
CTCCTGTGCAGTCTCTGGATTCACCTTCAGTAACTTTGCTATGCACTGGG13590.176665583360416No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG13460.17497562560935975No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT13250.17224569385765356No Hit
GCCTGAGACCGGGAGATACGGCTATGTACTATTGTGCGAGAGAGGGAGTC13190.17146571335716607No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG12500.16249593760155995No Hit
GTCTCAGGCTGTTCATTTGCAAAAACACCTTGTTTTTGGTGTTGTCTCTG12470.16210594735131623No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12280.1596360090997725No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC12170.15820604484887876No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA12050.1566460838479038No Hit
GTCTCTGGATTCACCTTCAGTAACTTTGCTATGCACTGGGTCCGCCAGGC11630.1511862203444914No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11580.15053623659408516No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11500.14949626259343518No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC11300.1468963275918102No Hit
GGGCTGGAGTGGGTGGCAGTTATATCGTCTGATGGAAGTGAGACATATTA11240.1461163470913227No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT11090.144166395840104No Hit
TGGTAGCACCAATCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCA11030.14338641533961652No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10820.1406564835879103No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10690.13896652583685407No Hit
CTCCGTGACGGACCGATTTACCATCTCCAGAGACAACACCAAAAACAAGG10680.13883652908677283No Hit
CTCCAGAGACAACACCAAAAACAAGGTGTTTTTGCAAATGAACAGCCTGA10530.1368865778355541No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10520.13675658108547287No Hit
TCCTATGACAGCAGCCTGAGGGCGGTGGTATTCGGCGGAGGGACCAAGCT10470.1361065973350666No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG10280.1336366590835229No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT10110.1314267143321417No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10030.1303867403314917No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9910.12882677933051673No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG9860.1281767955801105No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG9680.12583685407864803No Hit
GTACATAGCCGTATCTCCCGGTCTCAGGCTGTTCATTTGCAAAAACACCT9660.12557686057848555No Hit
CCCTGAGACTCTCCTGTGCAGTCTCTGGATTCACCTTCAGTAACTTTGCT9600.12479688007799804No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAGCACCAATCGGCCCT9480.12323691907702307No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC9450.12284692882677933No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG9360.12167695807604811No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA9210.11972700682482938No Hit
CTACAGACTCCCTCTCTCGCACAATAGTACATAGCCGTATCTCCCGGTCT9120.11855703607409815No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC9080.11803704907377315No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT9080.11803704907377315No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9030.11738706532336693No Hit
ACTACAGACTCCCTCTCTCGCACAATAGTACATAGCCGTATCTCCCGGTC8740.11361715957101072No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC8700.11309717257068574No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC8690.11296717582060449No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT8660.11257718557036074No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8630.11218719532011699No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC8580.11153721156971076No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8520.11075723106922326No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC8500.11049723756906078No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8380.1089372765680858No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8380.1089372765680858No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT8370.10880727981800456No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8370.10880727981800456No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT8360.1086772830679233No Hit
ATATAACTGCCACCCACTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAG8360.1086772830679233No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8300.10789730256743581No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA8290.10776730581735458No Hit
GTGTCAGGTCCACCTGGTGGAGTCTGGGGGAAGCGTGGTCCAGCCTGGGA8260.10737731556711083No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGACAGAGGGTCAC8250.10724731881702958No Hit
GCTATGTACTATTGTGCGAGAGAGGGAGTCTGTAGTGGTGCAACCTGTTA8050.10464738381540462No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACCGCCCTCAGGC8040.10451738706532337No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT8020.10425739356516087No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG7860.10217744556386091No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCAAAGTTAC7770.10100747481312966No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGT7750.10074748131296718No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8300.073.712441
GGTATCA2700.071.978031
ATCAACG8850.066.6915053
TCAACGC9000.065.579994
CAACGCA9500.062.1284075
TATCAAC9600.061.481232
AACGCAG9900.059.6181646
CGCAGAG11550.051.1012848
ACCTACG450.00991736847.9853551
GCAGAGT14950.040.442519
CAGCGCT4450.040.4370969
GGCAGCG4950.039.2607427
TACATGG17100.038.725022
GTACATG17900.036.9942971
ACATGGG17950.036.0892643
TACACCG850.002840446833.8720135
ACGCAGA17450.033.823497
TATTGAG8000.032.3901145
CCTAGTA900.003758361831.9902382
CTATAAG1801.376302E-731.9902381
Produced by FastQC (version 0.11.5)