FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279849

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279849
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947963
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCGGACCGGAGCCCACTGACGGCCAGGGAGG20000.21097869853570236No Hit
GATTATTACTGTGCAGCATGGGATGACAGGTTGGGTGGTCTGGCGTTCGG19390.20454384823036342No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12140.12806407001117132No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC12120.12785309131263561No Hit
CACTAATACTCAGCGGCCCTCAGGGGTCTCTGACCGATTCTCTGCCTCCA12010.12669270847068925No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCAG11770.12416096408826083No Hit
CTAATACTCAGCGGCCCTCAGGGGTCTCTGACCGATTCTCTGCCTCCAAG11080.11688219898877909No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10820.11413947590781498No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA10770.11361202916147571No Hit
GTATGGAACAGTAAACTACCCACGGAAGTTTAAGGGCAGACTCACGATTA10240.10802109365027959No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10210.10770462560247605No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9720.10253564748835134No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA9480.1000039031059229No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)