FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences947963
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGGTTGGGTGGTCTGGCGTTCGG19350.20412189083329202No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC13160.13882398363649215No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13080.13798006884234934No Hit
CACTAATACTCAGCGGCCCTCAGGGGTCTCTGACCGATTCTCTGCCTCCA12750.13449892031651023No Hit
CTAATACTCAGCGGCCCTCAGGGGTCTCTGACCGATTCTCTGCCTCCAAG10400.10970892323856522No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA9890.1043289664259048No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCAG9650.10179722204347638No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12050.059.0980571
ATCAACG12700.054.348263
TCAACGC12600.054.211844
CAACGCA13300.051.358595
AACGCAG14150.048.7506186
GGTATCA6150.044.4460871
TATCAAC15250.044.319942
ACGCAGA16850.040.6070147
CGCAGAG16750.040.417318
GTACATG24000.034.1679271
GCAGAGT21100.032.4261559
TACATGG26400.031.5933132
ACATGGG25350.029.7803253
CAGAGTA20350.027.6047959
CAGCGCT5350.024.2310969
ATACACG8100.023.079664
GTACACG5650.022.906845
CATGGGG16100.020.5434344
GTGCTAG3901.3345925E-820.288651
TACACCC8200.020.167645
Produced by FastQC (version 0.11.5)