FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences776400
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAGATCTCAGGGTGTTCAGCTGCAGAAAGG23160.2982998454404946No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC18300.23570324574961357No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15030.19358578052550232No Hit
GTCTGGGGCTGAGGTGAGGAAGCCTGGGTCCTCGATGAAGGTCTCCTGCA14950.19255538382277176No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14630.18843379701184956No Hit
TACTAGTGGTTATCACAACTGGTTCGACCCCTGGGGCCAGGGAACCCTGG14350.18482740855229265No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13510.17400824317362185No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12950.166795466254508No Hit
GTGTATTACTGTGCGAGGGGGCCCCCATTTTACCATAATACTAGTGGTTA12750.16421947449768162No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12250.15777949510561565No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11990.15443070582174137No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11840.1524987120041216No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCAAATGATCACCA11760.15146831530139104No Hit
GTCCTGGTCATCTATTATGATAGCGACCGGCCCTCAGGGATCCCTGAGCG11320.145801133436373No Hit
CCATTACCTGTGGGGGAAACAATTTTGGAAGTAAAAGTGTCCACTGGTAC11270.1451571354971664No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10740.13833075734157652No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10720.13807315816589388No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG10500.13523956723338484No Hit
CCACTAGTATTATGGTAAAATGGGGGCCCCCTCGCACAGTAATACACGGC10180.13111798042246264No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9950.12815558990211232No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACACCAA9700.12493560020607934No Hit
CCCCTGGACAAGGGCTTGATTGGATGGGAGGGATCATCCCTCTCTTTGGT9620.12390520350334878No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9600.12364760432766615No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9570.12326120556414219No Hit
GTATTACTGTGCGAGGGGGCCCCCATTTTACCATAATACTAGTGGTTATC9530.12274600721277691No Hit
CTACTATCCCACACCTGACAGTAGTAGTCGGCCTCATCCCCGGCTTCGAC9450.12171561051004638No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9430.12145801133436372No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC9390.12094281298299844No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8860.11411643482740855No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8710.11218444100978876No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC8460.10896445131375579No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8430.10857805255023184No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8350.10754765584750128No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8310.107032457496136No Hit
CAGTAATACACGGCCGTGTCCTCAGATCTCAGGGTGTTCAGCTGCAGAAA8300.10690365790829469No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT8210.10574446161772282No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8210.10574446161772282No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG8160.10510046367851622No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA8070.10394126738794435No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA7920.10200927357032458No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7920.10200927357032458No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7800.10046367851622875No Hit
ATACACGGCCGTGTCCTCAGATCTCAGGGTGTTCAGCTGCAGAAAGGCTG7790.10033487892838742No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG7790.10033487892838742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)