FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757099
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAGACGGCCGTGTCCTCGGCTCTCAGACTGTTCATTTGCAGATACA22260.2940170307978217No Hit
GTAATAATGTTGGCAACGAAAATAATGTTGGCAACGGAGGAGCAACTTGG14380.189935530227883No Hit
GTACCAGGGCCACCCTCCCAAACTCCTCTCCTACAGGAATAACAACCGGC13900.1835955403454502No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13810.18240679224249404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13070.17263264117374347No Hit
GTCTACTACTGTGCGAAAGAGGCCAGGGCTTCTGCCAGTGACTGGTACAG12360.16325473947264493No Hit
GTCCCATGCTGAGCAGTAATAATCAGCCTCGTCCTCAGGCTGGAGTCCAG11810.15599016773235733No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAGGCGCTGAGG11800.15585808460980663No Hit
TCCACAGACAGCGCCAAGAATTCACTGTATCTGCAAATGAACAGTCTGAG11470.15149934156563408No Hit
CACCTGCACTGGGAACAGTAATAATGTTGGCAACGAAAATAATGTTGGCA11470.15149934156563408No Hit
GTGCAGTACCAGGGCCACCCTCCCAAACTCCTCTCCTACAGGAATAACAA11020.14555560105085333No Hit
GTAATAATCAGCCTCGTCCTCAGGCTGGAGTCCAGCAATGGTCAGGGAGG10830.14304602172239034No Hit
GCATGGGACAGCAGCCTCAGCGCCTGGGTGTTCGGCGGAGGGACCAAGCT10700.14132894112923144No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9700.1281206288741631No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9590.12666771452610556No Hit
GTGTAGGCTATGGATTTACCTTCAGTGATCAATGGATGAACTGGGTCCGT9510.1256110495457001No Hit
GTCTGTGGAAATGGTGAATCGGCCCTTCACAGAGTCCACATAATTTGCCC9280.12257313772703438No Hit
GATTATTACTGCTCAGCATGGGACAGCAGCCTCAGCGCCTGGGTGTTCGG9050.11953522590836865No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT8230.10870440985921259No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8080.10672316302095236No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7990.1055344149179962No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG7980.10540233179544552No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA7970.10527024867289483No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT7890.10421358369248936No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC7710.10183608748657706No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6200.068.167081
GTATCAA17300.067.9066241
ATCAACG19650.058.230913
TCAACGC20300.056.35524
TATCAAC21450.053.3726232
CAACGCA21600.053.2944765
CACCGAC1850.050.233757
CGCAGAG24050.047.2613378
GTAGACG6800.045.2136544
ACCGACC1900.045.149368
TAGACGG6900.044.558385
GACGGCC7200.040.7075847
AACGCAG29100.039.7966046
GCAGAGT29450.038.5801289
GTGCTAG4350.037.8750271
ACGCAGA30550.037.6737257
GCACCGA1951.9826984E-1036.659746
GTGTAGA4200.035.8166051
AGACGGC8200.035.7432446
TTTCGCG1052.0177958E-434.0276959
Produced by FastQC (version 0.11.5)