FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912076
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCTCAGATCTCAGGCTACTCAGTTCCAAGTAGG28900.31685955994895165No Hit
GTCTATTACTGTGCGAGAGAGCAGTGGGTGGCCACCGGACGCCTCCGTCC20210.22158241199198314No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA16570.18167345703647503No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13230.14505370166521211No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT12900.14143558212254242No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12810.14044882224726887No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12660.1388042224551463No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12370.1356246628570426No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12020.13178726334208993No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCGAACCACTC11390.12487994421517504No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11290.12378354435375999No Hit
CCCCTGGACAGGGGCTTGAGTGGATGGGAAGAATCATCCCTGTCTTTGGA11080.12148110464478838No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10670.11698586521298664No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT10290.11281954573960941No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9850.10799538634938316No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9460.10371942688986444No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG9380.10284230700073241No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6000.080.010621
GTATCAA14500.073.6278841
ATCAACG19600.053.2791863
TCAACGC19800.053.0702254
CAACGCA20050.052.4084975
AACGCAG21400.049.0996636
TATCAAC21650.048.9272422
CAGCGCT4650.044.565729
CGCAGAG26400.040.3419888
TAGTACG1651.5679689E-938.9903684
CCGTACC951.1184644E-437.6202248
ACGCAGA28050.037.459287
TAGACGG12150.035.8882525
ATAGACG12800.034.624264
AGACGGC13000.032.990046
GTAATAG14450.032.7265471
AATAGAC14400.031.2923983
CAGAGTA28950.030.8544169
GCAGCGC7000.029.612498
GTACATG22400.029.1083431
Produced by FastQC (version 0.11.5)