FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279860

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279860
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1277624
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC51320.40168312429948094No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC39960.31276807574059345No Hit
GACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATACCAGAGTACTGTCG36860.2885042860810379No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCCGTGACGTT36110.28263401438921No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATACCAG32180.2518737907240315No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC31180.24404676180159424No Hit
GATTATTACTGCAGCTCATATGCAAGCGACAGTACTCTGGTATTCGGCGG29660.23214967783948956No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC28430.2225224322648917No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT28320.2216614590834236No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT28260.22119183734807737No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA27500.21524329536702505No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG26720.20913821280752398No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA26490.20733799615536339No Hit
GTAATAATCAGCCTCGTCCTCAGATTGGAGCCCAGAGATGGTCAGGGAGG25370.19857172376223364No Hit
CTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC24960.19536264190403435No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT24650.1929362629380788No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23720.18565712604021214No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA23370.1829176659173591No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG23140.18111744926519852No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22570.17665604277940927No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT22480.1759516101763899No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA22170.17352523121043437No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT21330.16695052691558704No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC20940.16389798563583652No Hit
ACCTAGGACGGTCAGCTTCGTCCCTCCGCCGAATACCAGAGTACTGTCGC20880.1634283639004903No Hit
TCTCTGGGCTCCAATCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT20470.160219282042291No Hit
GGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGCACAGGGTCCT20460.16014101175306664No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT20010.15661884873796986No Hit
CTCCTGCACTGGAACCAGCCGTGACGTTGGTAATTATAACTATGTCTCCT19750.15458382121813616No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC19590.1533314965905462No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT19200.15027895531079566No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA18940.14824392779096196No Hit
TTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAAAC18840.14746122489871824No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG17930.14033862857930035No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA17300.13540760035816485No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG16760.13118100474004873No Hit
GTAATACATGCCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG15990.12515419246977202No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC15970.12499765189132328No Hit
GTACTCTGGTATTCGGCGGAGGGACGAAGCTGACCGTCCTAGGTCAGCCC15940.12476284102365014No Hit
GCTCATATGCAAGCGACAGTACTCTGGTATTCGGCGGAGGGACGAAGCTG15930.12468457073442576No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15740.1231974352391627No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGT15610.12217992147924585No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15590.12202338090079712No Hit
TCTCCACCATGGCCTGGGCTCTGCTATTCCTCACCCTCCTCACTCAGGGC15260.1194404613563928No Hit
CATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGATTGGAGCCCAGAG15130.11842294759647595No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG14850.11623137949819352No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC14840.11615310920896915No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG14640.11458770342448168No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14490.11341364908611609No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG14400.11270921648309676No Hit
CCAGAGTACTGTCGCTTGCATATGAGCTGCAGTAATAATCAGCCTCGTCC14390.11263094619387239No Hit
ATGTATTACTGTGCGAGATCCAATAGAGATGGCTACAATTACAGGTCTTT14140.11067418896326306No Hit
GTCTGGAGCAGAGGTGAAGAAGTCCGGAGAGTCTCTGAAGATCTCTTGTA14060.11004802664946807No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA13800.10801299912963438No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13300.10409948466841575No Hit
GTCGATCACCATCTCCTGCACTGGAACCAGCCGTGACGTTGGTAATTATA13150.10292543033005015No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG13120.10269061946237704No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)