FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279861

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279861
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences683066
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG25630.37521996410302955No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG24360.35662732444595396No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC20250.29645744335100854No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG20150.29499345597643567No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT20000.29279747491457636No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG16310.23877634079283702No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAGTGTTCCTG15230.22296527714744988No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC15220.2228188784099926No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT15050.2203300998732187No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT12840.187975978895158No Hit
GTATACAGCCGTGTCCTCGGCTCTCAGTCTGTTCATTTGCAGATACAGCG12420.1818272319219519No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGTAAAGAAGTT12210.17875285843534885No Hit
GCTTGATACCACTGCTTGATACCACTGCTTGATACCACTGCTTGATACCA12000.1756784849487458No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT11820.17304330767451462No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11530.16879774428825325No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCA11520.16865134555079597No Hit
GATTAAGACTGATGGGAGTGTAACAAGCTACGCGGACTCCGTGAAGGGCC10590.15503626296726816No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG10490.1535722755926953No Hit
GTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAGTGGTATCAAGCAG10380.15196188948066514No Hit
GTAATAATCAGCCTCGTCTTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG10100.14786272483186105No Hit
GCTGTATACTTCTGCGCACGAGATCTCAGGGGATCCAACTACTGGGGCCA10010.14654513619474546No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9480.1387860031095092No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA9350.13688281952256443No Hit
GAAGTATACAGCCGTGTCCTCGGCTCTCAGTCTGTTCATTTGCAGATACA9340.13673642078510714No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC9300.136150825835278No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9220.13497963593561968No Hit
CTCCTGCACTGGAACCAGTAAAGAAGTTGGTGGTTATGACTATGTCTCCT9220.13497963593561968No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCCAAGTGTTCCTGT9030.13219805992393122No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8940.13088047128681563No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT8920.13058767381190103No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC8780.128538091487499No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC8740.12795249653766985No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTGACTCAGCCT8580.12561011673835326No Hit
TCTCTGGGCTCCAGGCTGAAGACGAGGCTGATTATTACTGCAGCTCATCT8550.1251709205259814No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8290.12136455335209188No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC8190.11990056597751901No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAATCTGCCCTG8190.11990056597751901No Hit
CCCTGAGGAGACGGCGACCAGGGTTCCCTGGCCCCAGTAGTTGGATCCCC8190.11990056597751901No Hit
GATTATTACTGCAGCTCATCTACAAACAGGAACACTTGGGTTTTCGGCGG8130.11902217355277528No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8110.1187293760778607No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG8100.11858297734040342No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC8030.11755818617820239No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA7930.1160941988036295No Hit
GTCCTGGGCCCAATCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC7900.11565500259125765No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC7900.11565500259125765No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG7850.1149230089039712No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT7780.1138982177417702No Hit
GTATACTTCTGCGCACGAGATCTCAGGGGATCCAACTACTGGGGCCAGGG7710.11287342657956918No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCGGTGACTACTGGATGCACTGGA7680.11243423036719731No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC7470.10935985688059426No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA7400.10833506571839323No Hit
GTCTTAATCTGTGAGACCCATTCCGGCCCCTTCCCTGGGGGTTGGCGGAT7380.10804226824347868No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7350.1076030720311068No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7200.10540709096924748No Hit
CTCCAGAGACAACGCCAAGAACACGCTGTATCTGCAAATGAACAGACTGA7150.10467509728196103No Hit
GTGTGAGGTGCAGCTGGTGGAGTCCGGGGGAGGCTTAGTTCAGCCTGGGG7140.10452869854450375No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA7060.10335750864484544No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC7050.10321110990738816No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG7030.10291831243247357No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA6990.10233271748264441No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG6970.10203992000772985No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG6860.1004295338956997No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATACG3150.052.566299
GTGCTAG4150.045.199981
CAGAGCG8950.039.415159
GTCATAG9550.039.2837561
ATAGGTG9700.038.5941734
ATCAACG23700.038.5785143
TAGGTGG9950.038.348025
CAACGCA23800.038.1139265
AACGCAG23950.037.8752176
TCAACGC23850.037.7321664
GTGGTCA10050.037.2500958
GTGTAGA2600.036.0730631
CTAGAGA4400.035.9964874
TGCTAGA5450.035.69762
GCTAGAG4450.035.592033
CGCAGAG25700.035.0160378
ACGCAGA25850.034.8128477
TCTACAC5300.033.958953
AGAATAC5100.033.8790448
AGGTGGT12050.032.262416
Produced by FastQC (version 0.11.5)