FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009748
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACAGCCGTGTATTC29170.28888395916604936No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26060.25808419526456106No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA25450.25204308401700226No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT24560.24322900367220335No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC23590.2336226464424787No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC23500.23273133494693723No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCGTGGTCCCTCCGCCGAAAACCCA23430.23203809267262726No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23280.23055257351339148No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA22610.22391725460213838No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT22170.21955973173504675No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC21670.21460800120426085No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT21150.2094582014522435No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG19980.19787115201020453No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG18330.18153044125861106No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18060.17885650677198667No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT17970.17796519527644522No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17100.16934918415287775No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC17050.16885401109979914No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTACAAGTATGTCTCCT16980.16816076882548914No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA16720.16558586894948046No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16500.16340710751593465No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT16080.1592476538700745No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCTGCTCATAT15940.15786116932145444No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT15680.1552862694454458No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG15620.1546920617817515No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT14980.14835384670234555No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC14700.14558087760510544No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14690.1454818429944897No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14580.14439246227771682No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG14460.1432040469503282No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14370.14231273545478673No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG14040.13904459330446806No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13850.13716293570276938No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13700.13567741654353363No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA13620.1348851396586079No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT13610.13478610504799218No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT13450.1332015512781407No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG13270.13141892828705776No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13260.13131989367644203No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA13020.1289430630216648No Hit
CCATGTAGGCTGTGCTCGAGGATTCGTCCGCGGTGATCGTGAGTCTGCCC12990.12864595918981764No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12950.12824982074735478No Hit
CAGCTATGGCTGGGGGTGTTTCTTATGTCAGGGGCCGATACTACTTTGAC12900.1277546476942762No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12890.1276556130836605No Hit
GGAATACACGGCTGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG12830.12706140541996616No Hit
CATGTAGGCTGTGCTCGAGGATTCGTCCGCGGTGATCGTGAGTCTGCCCT12830.12706140541996616No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCTTCCCCATCTTTGGT12730.126071059313809No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTG12710.12587299009257755No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12610.12488264398642038No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG12470.12349615943780033No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC12350.12230774411041172No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12320.12201064027856454No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT12190.12072319034056023No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTC11920.11804925585393584No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11910.11795022124332012No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11900.11785118663270439No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11840.11725697896901009No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11700.11587049442039003No Hit
GTGTATTCCTGTGCGAGAGTTGTGGCCGTGGACACAGCTATGGCTGGGGG11580.11468207909300142No Hit
GCTATGGCTGGGGGTGTTTCTTATGTCAGGGGCCGATACTACTTTGACTT11500.1138898022080757No Hit
GACCCAGGACGGTCAGCGTGGTCCCTCCGCCGAAAACCCACATGTAGCTG11480.11369173298684424No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC11340.11230524843822418No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG11260.11151297155329845No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT11200.11091876388960413No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC11200.11091876388960413No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC11130.11022552161529411No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTACA11130.11022552161529411No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11120.11012648700467839No Hit
ACCCAGGACGGTCAGCGTGGTCCCTCCGCCGAAAACCCACATGTAGCTGC11090.10982938317283125No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10990.10883903706667407No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG10970.10864096784544264No Hit
ACATGGAGCTGAGCAGCCTGAGATCTGAGGACACAGCCGTGTATTCCTGT10860.10755158712866973No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCT10790.10685834485435969No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10700.10596703335881824No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGT10680.10576896413758681No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT10600.10497668725266106No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10570.1046795834208139No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC10480.10378827192527244No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10460.10359020270404101No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA10410.10309502965096243No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG10250.10151047588111094No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC10210.10111433743864805No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT10210.10111433743864805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5850.092.4654161
GTATCAA17800.075.769691
ATCAACG19850.066.8247453
TATCAAC20150.066.545382
TCAACGC20400.064.6697164
CAACGCA21200.062.2293555
AACGCAG22350.059.282336
CGCAGAG25100.053.053118
GCAGAGT27400.048.5997479
ACGCAGA29950.044.2215357
CAGCGCT5900.042.7000969
GAATACG1704.1836756E-1142.3412749
GTACATG27000.035.5272671
GCAGCGC7800.035.067268
ACATGGG28050.033.668093
AGCGCTG6100.033.0908785
CTAGAGA2851.0913936E-1130.3540364
GGCAGCG9000.028.7920677
CATGGGG18800.028.7596444
TCTACAC4850.028.2417683
Produced by FastQC (version 0.11.5)