FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009748
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25600.25352860317623804No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCGTGGTCCCTCCGCCGAAAACCCA25190.2494681841409936No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24950.24709135348621636No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACAGCCGTGTATTC24690.24451645361020768No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA22860.2263931198675313No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21360.21153792827517362No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT21320.21114178983271076No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC21120.2091610976203964No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20570.20371419403653188No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT20340.20143639799237037No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18860.1867792756212441No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT18560.18380823730277257No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG18500.18321402963907826No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16350.1619215883566989No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG16230.16073317302931028No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15530.15380075028621001No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15500.15350364645436287No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTACAAGTATGTCTCCT15410.15261233495882143No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC14950.1480567428704984No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTTCTGCTGCTCATAT14830.14686832754310974No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14650.14508570455202685No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14510.1436992200034068No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14490.14350115078217535No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA14240.1410252855167824No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG13820.13686583187092224No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA13810.13676679726030652No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13510.13379575894183499No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG13230.1310227898445949No Hit
TCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13130.1300324437384377No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC12970.12844788996858622No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCT12950.12824982074735478No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12760.12636816314565616No Hit
CCATGTAGGCTGTGCTCGAGGATTCGTCCGCGGTGATCGTGAGTCTGCCC12690.12567492087134613No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG12390.12270388255287458No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12350.12230774411041172No Hit
GCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCTGGCAACACGG12340.122208709499796No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12280.12161450183610167No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12260.12141643261487024No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12260.12141643261487024No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12260.12141643261487024No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11930.11814829046455155No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11530.11418690603992283No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT11380.11270138688068707No Hit
CATGTAGGCTGTGCTCGAGGATTCGTCCGCGGTGATCGTGAGTCTGCCCT11250.11141393694268273No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC11180.1107206946683727No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11070.1096313139515998No Hit
GGAATACACGGCTGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG11020.10913614089852121No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTG10990.10883903706667407No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCTTCCCCATCTTTGGT10950.10844289862421119No Hit
CAGCTATGGCTGGGGGTGTTTCTTATGTCAGGGGCCGATACTACTTTGAC10830.10725448329682258No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10830.10725448329682258No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC10770.10666027563312826No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10710.10606606796943396No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTACA10700.10596703335881824No Hit
GACCCAGGACGGTCAGCGTGGTCCCTCCGCCGAAAACCCACATGTAGCTG10690.10586799874820253No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT10580.10477861803142963No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCT10540.10438247958896676No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT10490.10388730653588815No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10470.10368923731465672No Hit
GCTCATATGCAGGCAGCTACATGTGGGTTTTCGGCGGAGGGACCACGCTG10380.10279792581911527No Hit
GTGTATTCCTGTGCGAGAGTTGTGGCCGTGGACACAGCTATGGCTGGGGG10320.10220371815542095No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10230.10131240665987948No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG10100.10002495672187517No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA10100.10002495672187517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10400.070.545591
GGTATCA3600.063.9367451
ATCAACG11850.061.2881743
TCAACGC11800.060.938484
TATCAAC12250.059.2898672
CAACGCA12800.055.60765
AACGCAG13450.052.872996
CAGCGCT6400.046.136719
CGCAGAG15750.045.2686358
GCAGAGT17500.040.330249
GCAGCGC8550.037.062048
ACGCAGA20450.035.2167637
GGCAGCG8800.035.190757
GTACATG22350.031.5392681
TATTGAG14700.030.323785
GAGCGCT950.00488398230.3234867
ACATGGG23400.028.8858833
GTACGGA1000.006313767328.7715341
TTATTGA15100.028.5724814
CTAGAGA3750.026.8454324
Produced by FastQC (version 0.11.5)