FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044759
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT30850.29528340985815865No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA23140.22148648635714074No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC22010.2106705948453184No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC21650.20722482409818915No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC20250.19382460452601988No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19830.18980453865436908No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18690.17889293128845984No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17830.1706613678369844No Hit
GTCTCTGGCGGCTCCATCAGCAGCAATATCTGGTGGAGCTGGGTCCGGCA17300.16558842757037748No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCGCCATGTCAGTGGACAAGTCC17280.16539699586220363No Hit
GCATAATTCTTACCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCA17200.16463126902950825No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16920.1619512251150744No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT16740.16022833974150977No Hit
ATTCTTACCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA16170.15477253605855512No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG16110.1541982409340336No Hit
ACTTATTACTGTTCACAGCATAATTCTTACCCATTCACTTTCGGCCCTGG15170.1452009506498628No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG15130.1448180872335151No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG14640.14012801038325584No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC14480.13859655671786508No Hit
GAATTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTGCCTGAT13800.13208787863995428No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT13790.13199216278586737No Hit
TTCTGAGACTCATGGACCTCCTGCACAGGAACATGAAACACCTGTGGTTC13580.12998212985004198No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT13390.12816352862239044No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG13300.12730208593560813No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC13250.1268235066651735No Hit
GATCTATTCTGCATCCAGATCGCAAAGTGGGGTCCCATCAAGGTTCAGCG13200.1263449273947389No Hit
GAAATAGACGGCCGTGTCCGCGGCGGTGACAGACGTCAAGTTAAGGGAGA12860.12309058835578347No Hit
GAATTATGCTGTGAACAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCT12820.12270772493943578No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG12580.12041054444134963No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12520.11983624931682808No Hit
AGATACGACTATGGTGACCACAACTTGTTCGACCCCTGGGGCCATGGAAT12520.11983624931682808No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12410.11878337492187194No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12370.11840051150552423No Hit
ATTCTGCATCCAGATCGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGT12350.11820907979735039No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12150.11629476271561193No Hit
GTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGA12010.114954740758395No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11720.11217898098987422No Hit
GTCTCCATCCTCCCTGTCGGCGTCTGTGGGAGACAGAGTCACCATCACTT11670.11170040171943961No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG11360.10873321024274499No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG11360.10873321024274499No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA11270.10787176755596267No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT11200.10720175657735419No Hit
GGATAGGGGAAATCTATCATTCTGGGAGGATCAACTACAACCCGTCCCTC11170.10691460901509342No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG11060.10586173462013726No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10970.10500029193335496No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10950.10480886022518111No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC10880.10413884924657264No Hit
GGTAAGAATTATGCTGTGAACAGTAATAAGTCGCAAAATCTTCAGGCTGC10870.10404313339248572No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10680.10222453216483418No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10640.10184166874848649No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT10600.1014588053321388No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10570.10117165776987802No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG10550.10098022606170418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)