FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279866

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279866
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044759
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29620.2835103598054671No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22010.2106705948453184No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21440.20521479116236374No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC19430.18597590449089216No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19290.18463588253367522No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18440.17650003493628674No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA18420.1763086032281129No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16610.15898403363837976No Hit
GCATAATTCTTACCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCA16560.15850545436794516No Hit
GTCTCTGGCGGCTCCATCAGCAGCAATATCTGGTGGAGCTGGGTCCGGCA15850.15170962872777358No Hit
ACTACAACCCGTCCCTCAAGAGTCGAGTCGCCATGTCAGTGGACAAGTCC15290.14634954089890587No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15280.14625382504481896No Hit
ATTCTTACCCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAACGA14860.14223375917316816No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14490.138692272571952No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13960.13361933230534506No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC13490.12912068716325967No Hit
ACTTATTACTGTTCACAGCATAATTCTTACCCATTCACTTTCGGCCCTGG13470.12892925545508582No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13380.1280678127683035No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13260.12691922251926044No Hit
GAATTAGGCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGTGCCTGAT13190.12624921154065197No Hit
GATCTATTCTGCATCCAGATCGCAAAGTGGGGTCCCATCAAGGTTCAGCG12750.12203771396082733No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG12670.12127198712813193No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12660.12117627127404501No Hit
GTAATAAGTCGCAAAATCTTCAGGCTGCAGGCTGCTGATTGTGAGAGTGA12580.12041054444134963No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA12500.11964481760865424No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT12450.11916623833821963No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12160.11639047856969885No Hit
GAATTATGCTGTGAACAGTAATAAGTCGCAAAATCTTCAGGCTGCAGGCT12080.11562475173700346No Hit
TTCTGAGACTCATGGACCTCCTGCACAGGAACATGAAACACCTGTGGTTC11990.11476330905022115No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11920.11409329807161268No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11690.11189183342761345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11630.11131753830309192No Hit
ATTCTGCATCCAGATCGCAAAGTGGGGTCCCATCAAGGTTCAGCGGCAGT11560.11064752732448345No Hit
GAAATAGACGGCCGTGTCCGCGGCGGTGACAGACGTCAAGTTAAGGGAGA11520.11026466390813576No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG11170.10691460901509342No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC11170.10691460901509342No Hit
GGATAGGGGAAATCTATCATTCTGGGAGGATCAACTACAACCCGTCCCTC11060.10586173462013726No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11010.10538315534970265No Hit
GTCTCCATCCTCCCTGTCGGCGTCTGTGGGAGACAGAGTCACCATCACTT10880.10413884924657264No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG10870.10404313339248572No Hit
AGATACGACTATGGTGACCACAACTTGTTCGACCCCTGGGGCCATGGAAT10750.10289454314344265No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10660.10203310045666034No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10530.10078879435353033No Hit
GGTAAGAATTATGCTGTGAACAGTAATAAGTCGCAAAATCTTCAGGCTGC10510.10059736264535649No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)