FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279867

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279867
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931860
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACATGGAGCTGAGCGGCCTGACATCTGACGACACGGCCGTCTTCTT30770.3301998154229176No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC28380.3045521859506793No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT22910.2458523812589874No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC22750.24413538514369112No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT22250.2387697722833902No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTAGCTG22150.23769664971133No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA21510.2308286652501449No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA21290.22846779559161245No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT21160.22707273624793423No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC20250.2173073208421866No Hit
GTAATAATCAGTCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG20110.21580494924130236No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT20040.21505376344086022No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTACACTTGGGTGTTCGGCGG19720.21161977121026765No Hit
GTCTGGGGCTGAAGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA17940.19251818942759644No Hit
CCTATCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGG17790.19090850556950614No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG17340.18607945399523532No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG16650.1786749082480201No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG16230.17416779344536734No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16000.17169961152962893No Hit
CATGTAGGCTGTGCCCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT15750.16901680509947847No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15740.16890949284227244No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA15550.1668705599553581No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAA15490.16622668641212202No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT15420.16547550061167987No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCT15120.16225613289549934No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14840.15925138969373082No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14830.1591440774365248No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG14820.15903676517931878No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC14760.15839289163608267No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14730.15807095486446462No Hit
CCATGTAGGCTGTGCCCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCC14660.15731976906402248No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT14390.15442233811946002No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT14210.15249071748975168No Hit
GAAGAAGACGGCCGTGTCGTCAGATGTCAGGCCGCTCAGCTCCATGTAGG14040.15066640911724938No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAATCTGCCCTGACTCAGCCT13780.1478762904298929No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13710.14712510462945078No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGACTGATTATTACTGCTGCTCATAT13290.14261798982679802No Hit
ACATGGAGCTGAGCGGCCTGACATCTGACGACACGGCCGTCTTCTTCTGT13280.14251067756959201No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG12900.1384328117957633No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA12870.13811087502414526No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA12770.13703775245208508No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC12740.13671581568046703No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12650.13575000536561285No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12640.13564269310840682No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG12600.13521344407958277No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA12580.13499881956517074No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA12390.1329598866782564No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA12370.13274526216384436No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC12290.1318867641061962No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG12250.13145751507737213No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTGTCTTTGGT12020.12898933316163372No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAATCTGCCCTG11380.12212134870044856No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11310.12137016290000645No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC11260.12083360161397635No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT11190.12008241581353421No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTAGCTGC11170.11986779129912219No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11110.11922391775588607No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT11060.11868735646985598No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11040.11847273195544396No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10970.11772154615500181No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC10690.11471680295323332No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10540.11310711909514304No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10470.11235593329470092No Hit
TCCCTGTCTTTGGTACACCAAAGTACGCACAGAAGTTCCAGGGCAGAGTC10450.11214130878028888No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10420.11181937200867083No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT10160.10902925332131437No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA10140.10881462880690232No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG10100.10838537977807824No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC10040.10774150623484216No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG9970.10699032043440002No Hit
TCTCTAAGCGGCCCTCAGGGGTCCCTGATCGCTTCTCTGGCTCCAAGTCT9830.10548794883351575No Hit
GTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATA9760.10473676303307364No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9670.10377095271821947No Hit
TCTCCACCATGGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGC9590.10291245466057133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9570.10269783014615928No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCT9550.10248320563174726No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC9470.10162470757409912No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC9460.1015173953168931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17600.073.638721
TATCAAC20950.060.486642
TCAACGC21050.059.817384
ATCAACG21350.059.0117263
CAACGCA21700.058.025625
AACGCAG22000.057.2251366
GGTATCA7000.055.237751
CGCAGAG25000.050.358128
CAGCGCT5300.040.4482049
ACGCAGA31850.039.527577
CTATACT2200.039.023434
GCAGCGC5850.035.4600228
TATTGAG11400.033.2611965
CTAGACT1153.4181596E-431.1056294
GTACATG25200.030.9717851
GAATACG1403.0317866E-530.625079
CAGAGTA27950.030.4241899
TCTATAC1453.8060578E-529.6214853
TTATTGA13100.029.4909884
ACATGGG26250.029.4522173
Produced by FastQC (version 0.11.5)