FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences931860
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTACATGGAGCTGAGCGGCCTGACATCTGACGACACGGCCGTCTTCTT27340.2933917112012534No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC27030.29006503122786687No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26480.2841628570815359No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT21160.22707273624793423No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20940.22471186658940184No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA20870.2239606807889597No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTAGCTG20490.21988281501513102No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTACACTTGGGTGTTCGGCGG20460.21956087824351297No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC19700.2114051466958556No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT19580.21011739960938336No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC18870.2024982293477561No Hit
GTAATAATCAGTCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG18550.1990642371171635No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTT18240.19573755714377694No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA17440.1871525765672955No Hit
GTCTGGGGCTGAAGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA16670.17888953276243214No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG16570.17781641019037195No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16080.17255810958727705No Hit
CCTATCACCTGGGTGCGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGG15350.16472431481123775No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG14660.15731976906402248No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT14600.1566758955207864No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA14460.15517352391990213No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14210.15249071748975168No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14130.15163221943210353No Hit
CCATGTAGGCTGTGCCCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCC13990.1501298478312193No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT13590.14583735754297855No Hit
CATGTAGGCTGTGCCCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT13480.14465692271371236No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT13440.14422767368488829No Hit
GGTTATAACTATGTCTCCTGGTACCAACAACACCCAGGCAAAGCCCCCAA13400.1437984246560642No Hit
CTCCTGCACTGGAACCAGCAGTGATGTTGGTGGTTATAACTATGTCTCCT13390.14369111239885818No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13390.14369111239885818No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13370.14347648788444617No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG13120.1407936814542957No Hit
GAAGAAGACGGCCGTGTCGTCAGATGTCAGGCCGCTCAGCTCCATGTAGG12960.13907668533899942No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12720.136501191166055No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG12660.13585731762281889No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12610.13532075633678878No Hit
TCCTCACTCAGGGCACAGGATCCTGGGCTCAATCTGCCCTGACTCAGCCT12460.1337110724786985No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGACTGATTATTACTGCTGCTCATAT11900.1277015860751615No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11790.1265211512458953No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG11670.12523340415942308No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11490.12330178352971477No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11450.12287253450089068No Hit
CCCCTGGACAAGGGCTTGAGTGGATGGGAGGGATCATCCCTGTCTTTGGT11400.12233597321486062No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11240.1206189770995643No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG11200.12018972807074024No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11110.11922391775588607No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT11040.11847273195544396No Hit
ACATGGAGCTGAGCGGCCTGACATCTGACGACACGGCCGTCTTCTTCTGT11020.1182581074410319No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTAGCTGC11010.11815079518382589No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10990.11793617066941386No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10710.11493142746764534No Hit
TCCTCAGCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAATCTGCCCTG10670.11450217843882128No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10660.11439486618161525No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10580.11353636812396711No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC10570.1134290558667611No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC10390.11149743523705279No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC10250.10999506363616852No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10190.10935119009293241No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC9970.10699032043440002No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9940.10666838366278197No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9860.10580988560513382No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG9710.10420020174704354No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9700.10409288948983753No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9610.10312707917498336No Hit
GGCCTGGGCTCTGCTCCTCCTCAGCCTCCTCACTCAGGGCACAGGATCCT9510.1020539566029232No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC9490.10183933208851115No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT9430.10119545854527504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10100.067.0108951
ATCAACG11050.060.5981863
TCAACGC11550.058.5982744
TATCAAC11750.057.600862
CAACGCA12050.056.1668135
AACGCAG12450.053.7781646
GGTATCA4000.048.6007231
GAATACG1551.6370905E-1146.4473239
CGCAGAG14750.044.9043248
CAGCGCT7350.044.077569
GGCAGCG7900.042.8314827
TGTACCG855.563299E-542.353575
GCAGCGC8100.040.8851138
TATTGAG9150.033.049675
ACGCAGA20900.031.6908537
GTACATG21550.031.0723881
CAGAGTA17000.030.914799
GTGCTAG2851.0913936E-1130.3162421
GTATAGA2151.9359504E-830.1399841
ACATGGG21500.030.1399823
Produced by FastQC (version 0.11.5)