FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340920
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATACAGAGCCGTGTCCACAGCTCTCAGGCTGTTCATTTGCAGATACA73020.5445515019538825No Hit
CTGTATCACTGTGCGAAAAGGGGGCTTGGCAGCGCCCAAGCTGAATACTT69130.5155415684753751No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGTACCTGGTGGAGTCTGGGGGAGGCG52440.39107478447632965No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT48720.3633326372937983No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT47690.35565134385347374No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC45670.3405870596307013No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT44710.33342779584166093No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC44430.3313396772365242No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA44260.3300718909405483No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA44010.32820749932881904No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC41550.3098618858694031No Hit
ATACAGAGCCGTGTCCACAGCTCTCAGGCTGTTCATTTGCAGATACAGCG38950.2904722131074188No Hit
GCTCTGTATCACTGTGCGAAAAGGGGGCTTGGCAGCGCCCAAGCTGAATA36410.2715299943322495No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG33990.25348268353071024No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG33150.24721832771529995No Hit
GTATCACTGTGCGAAAAGGGGGCTTGGCAGCGCCCAAGCTGAATACTTCC32080.23923873161709872No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT30950.23081168153208245No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG29330.21873042388807687No Hit
GGGCTGGAGTGGGTGGCAGTTATATCACATGATGGAAGAGAGAAATACTA28030.20903558750708467No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTCAGTAGCTATGCTATGCACTGGG27610.20590340959937953No Hit
CTCCAGAGACACCTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGA27240.20314411001402022No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC26810.1999373564418459No Hit
CAGTGATACAGAGCCGTGTCCACAGCTCTCAGGCTGTTCATTTGCAGATA26740.19941532679056173No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT26610.19844584315246247No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA25450.1897950660740387No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA25220.1880798257912478No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG24490.18263580228499834No Hit
GCCTGAGAGCTGTGGACACGGCTCTGTATCACTGTGCGAAAAGGGGGCTT24350.18159174298242997No Hit
GATGTACACTGGTATCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCGT24350.18159174298242997No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC23490.17517823583808131No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT23480.17510366017361215No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG23400.17450705485785878No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA23360.1742087521999821No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG23340.17405960087104377No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC23200.17301554156847537No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG23100.17226978492378367No Hit
GTGTCAGGTGTACCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGA22780.16988336366077023No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG22670.16906303135160933No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTCAGTAGCTATGCT22650.168913880022671No Hit
ATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG22620.1686901530292635No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC22550.1681681233779793No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT22000.16406646183217494No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT22000.16406646183217494No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT21930.1635444321808907No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT21490.16026310294424723No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC21310.15892074098380216No Hit
GTCCAGTGTCAGGTGTACCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC20900.15586313874056618No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC20850.15549026041822034No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC20680.15422247412224444No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20590.1535512931420219No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT19950.14877845061599498No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC19800.14765981564895744No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC19450.14504966739253647No Hit
CCTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGTGGAC19350.14430391074784477No Hit
CTTCCAGTATTGGGGCCCGGGCACCGTGGTCACCGTCTCCTCAGCCTCCA19290.14385645676102973No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTCA19000.1416937624914238No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA18910.14102258151120126No Hit
GTATCTGCAAATGAACAGCCTGAGAGCTGTGGACACGGCTCTGTATCACT18850.14057512752438625No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA18640.13900903857053368No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGCTAC18540.13826328192584195No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG18440.13751752528115027No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC18360.13692091996539688No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC18270.13624973898517437No Hit
GAGAAATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA18240.13602601199176687No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACACCT18190.13565313366942097No Hit
GCTCTCAGGCTGTTCATTTGCAGATACAGCGTGTTCTTGGAGGTGTCTCT18100.13498195268919846No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT18090.1349073770247293No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG18010.13431077170897593No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG17780.132595531426185No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTG17680.13184977478149332No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA17590.13117859380127075No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17220.12841929421591147No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT17190.12819556722250394No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA17020.12692778092652804No Hit
CCCCAGGAGTTCAGGTGCTGGGCACGGTGGGCATGTGTGAGTTTTGTCAC17010.12685320526205887No Hit
TCTCTGGAGATGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTATTTCTC16860.12573457029502133No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGTCATATGATGTACACTGGT16850.12565999463055216No Hit
TACTGGAAGTATTCAGCTTGGGCGCTGCCAAGCCCCCTTTTCGCACAGTG16720.12469051099245296No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC16620.12394475434776124No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA16520.12319899770306952No Hit
GCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACAC16320.12170748441368612No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA16230.1210363034334636No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG16220.12096172776899443No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCT16160.12051427378217941No Hit
CTCCAGCAGCTTGGGCACCCAGACCTACATCTGCAACGTGAATCACAAGC15930.11879903349938847No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA15920.1187244578349193No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15870.11835157951257345No Hit
GTCATAGGACTGGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAG15760.11753124720341257No Hit
CTCCTGGGGGGACCGTCAGTCTTCCTCTTCCCCCCAAAACCCAAGGACAC15730.11730752021000507No Hit
TCTCCAGAGACACCTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTG15600.11633803657190586No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC15420.11499567461146079No Hit
CCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCC15380.11469737195358409No Hit
GTGCATAGCATAGCTACTGAAGCTGAATCCAGAGGCTGCACAGGAGAGTC15090.11253467768397817No Hit
GTATTGGGGCCCGGGCACCGTGGTCACCGTCTCCTCAGCCTCCACCAAGG14770.11014825642096471No Hit
CTTTTAAGAGGTGTCCAGTGTCAGGTGTACCTGGTGGAGTCTGGGGGAGG14750.10999910509202637No Hit
GTGGGCATGTGTGAGTTTTGTCACAAGATTTGGGCTCAACTTTCTTGTCC14680.10947707544074219No Hit
TGCAGATACAGCGTGTTCTTGGAGGTGTCTCTGGAGATGGTGAACCGGCC14250.10627032186856784No Hit
GGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCG14180.10574829221728366No Hit
ATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTT14170.10567371655281449No Hit
TCCAGGAACAGCCCCCAAACTCCTCGTCTATGGTAACAGCAATCGGCCCT13990.10433135459236942No Hit
CCCAAGGACACCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGT13780.10276526563851685No Hit
TAGCTATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGT13680.10201950899382513No Hit
CCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGCTTGGGCACCCAGACC13650.10179578200041763No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA13510.10075172269784922No Hit
ATTGGGGCCCGGGCACCGTGGTCACCGTCTCCTCAGCCTCCACCAAGGGC13480.10052799570444172No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA16450.083.5977861
TCAACGC21250.063.58694
TATCAAC21150.063.27052
ATCAACG21450.063.0245823
CAACGCA22100.061.464755
TACCGAT350.003771590561.2730377
AACGCAG22400.060.3156436
CAGCGCT9450.051.417999
CGCAGAG26900.050.225678
GCAGAGT29500.046.264669
CACACCG700.001132647540.848697
GTATGTA21800.036.4691731
GTACATG30500.036.3991551
TATGTAC22050.036.0233762
ACATGGG31300.035.649693
TACATGG30950.035.6065672
GTACACG22100.035.5848545
TACACGG22400.035.1043436
ACACGGG22850.034.4130137
GAATACG1251.3975774E-534.298839
Produced by FastQC (version 0.11.5)