FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279870

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279870
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340920
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGATACAGAGCCGTGTCCACAGCTCTCAGGCTGTTCATTTGCAGATACA68370.5098738179757182No Hit
CTGTATCACTGTGCGAAAAGGGGGCTTGGCAGCGCCCAAGCTGAATACTT58960.4396981177102288No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT50280.3749664409509889No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGTACCTGGTGGAGTCTGGGGGAGGCG48700.36318348596485994No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT44200.3296244369537332No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA42680.3182889359544193No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC41210.3073263132774513No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT41200.30725173761298213No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA41140.3068042836261671No Hit
GCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC39090.2915162724099872No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC37430.27913671210810487No Hit
ATACAGAGCCGTGTCCACAGCTCTCAGGCTGTTCATTTGCAGATACAGCG37370.27868925812128986No Hit
GCTCTGTATCACTGTGCGAAAAGGGGGCTTGGCAGCGCCCAAGCTGAATA34570.2578080720699222No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG31080.23178116517018166No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG30050.2240998717298571No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG29310.21858127255913848No Hit
GTATCACTGTGCGAAAAGGGGGCTTGGCAGCGCCCAAGCTGAATACTTCC29160.21746263759210094No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT27740.20687289323747873No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA27640.20612713659278703No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT27360.20403901798765026No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC24760.18464934522566598No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTCAGTAGCTATGCTATGCACTGGG24530.18293410494287504No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG24500.1827103779494675No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT24120.17987650269963906No Hit
CAGTGATACAGAGCCGTGTCCACAGCTCTCAGGCTGTTCATTTGCAGATA24090.17965277570623153No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT23930.17845956507472482No Hit
GGGCTGGAGTGGGTGGCAGTTATATCACATGATGGAAGAGAGAAATACTA23910.17831041374578646No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG23770.1772663544432181No Hit
GATGTACACTGGTATCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCGT23270.17353757121975957No Hit
CTCCAGAGACACCTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGA23040.17182233093696864No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC22390.16697491274647258No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA22350.16667661008859588No Hit
GTGTCAGGTGTACCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCCTGGGA21560.16078513259553143No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC21350.15921904364167883No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG21090.15728007636548041No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC20860.1555648360826895No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT20640.15392417146436776No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTCAGTAGCTATGCT20360.15183605285923096No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA20330.15161232586582346No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT20260.1510902962145393No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC20210.15071741789219342No Hit
GTCCAGTGTCAGGTGTACCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC20100.14989708558303255No Hit
ATGCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG19900.14840557229364915No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT19620.14631745368851237No Hit
GCCTGAGAGCTGTGGACACGGCTCTGTATCACTGTGCGAAAAGGGGGCTT19230.14340900277421473No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC18990.14161918682695462No Hit
GCCTTGGGCTGACCAAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC18880.14079885451779375No Hit
TACTGGAAGTATTCAGCTTGGGCGCTGCCAAGCCCCCTTTTCGCACAGTG18830.14042597619544792No Hit
GCTCTCAGGCTGTTCATTTGCAGATACAGCGTGTTCTTGGAGGTGTCTCT18510.13803955493243444No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG18490.1378904036034961No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA18340.13677176863645854No Hit
CTCCAGCCCCTTGCCTGGAGCCTGGCGGACCCAGTGCATAGCATAGCTAC18100.13498195268919846No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT17940.13378874205769173No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG17850.13311756107746922No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT17670.13177519911702415No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG17540.13080571547892492No Hit
CTTCCAGTATTGGGGCCCGGGCACCGTGGTCACCGTCTCCTCAGCCTCCA17470.13028368582764074No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG17450.13013453449870238No Hit
GTATCTGCAAATGAACAGCCTGAGAGCTGTGGACACGGCTCTGTATCACT17430.12998538316976405No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC17370.129537929182949No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC17220.12841929421591147No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT17140.1278226889001581No Hit
GCCTGGGAGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTCA17010.12685320526205887No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA16710.12461593532798379No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC16470.12282611938072367No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA16400.12230408972943949No Hit
GAGAAATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGA16340.12185663574262448No Hit
CCTCCAAGAACACGCTGTATCTGCAAATGAACAGCCTGAGAGCTGTGGAC16290.12148375742027862No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC16280.12140918175580943No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG16270.12133460609134027No Hit
GTCTGGGGGAGGCGTGGTCCAGCCTGGGAGGTCCCTGAGACTCTCCTGTG16250.12118545476240193No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC16170.12058884944664858No Hit
ATACTACGCAGACTCCGTGAAGGGCCGGTTCACCATCTCCAGAGACACCT15920.1187244578349193No Hit
GTGCATAGCATAGCTACTGAAGCTGAATCCAGAGGCTGCACAGGAGAGTC15860.11827700384810429No Hit
CTCCTGCACTGGGAGCAGCTCCAACATCGGGTCATATGATGTACACTGGT15850.11820242818363512No Hit
CCCCAGGAGTTCAGGTGCTGGGCACGGTGGGCATGTGTGAGTTTTGTCAC15680.11693464188765922No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC15530.11581600692062166No Hit
CATTTACCCGGAGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG15510.11566685559168333No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA15150.11298213167079318No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC15140.11290755600632402No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT15080.112460102019509No Hit
GCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGTTCCACGACAC15040.11216179936163233No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA14810.11044655907884139No Hit
TCTCTGGAGATGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTATTTCTC14740.1099245294275572No Hit
GCACTGGAAGTCGCCGGTGTTTCCATTCGGTGATCAGCACTGAACACAGA14620.10902962145392715No Hit
CCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAGGACTACTTCCCC14590.10880589446051964No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA14270.10641947319750618No Hit
GTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTTCCCCTGCT13960.10410762759896192No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG13900.10366017361214687No Hit
GTATTGGGGCCCGGGCACCGTGGTCACCGTCTCCTCAGCCTCCACCAAGG13690.1020940846582943No Hit
GTGTAGTGGTTGTGCAGAGCCTCATGCATCACGGAGCATGAGAAGACGTT13690.1020940846582943No Hit
GAGGAAGACTGACGGTCCCCCCAGGAGTTCAGGTGCTGGGCACGGTGGGC13570.10119917668466427No Hit
GTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCCCCCAGGAGTT13550.10105002535572592No Hit
GCCTCATGCATCACGGAGCATGAGAAGACGTTCCCCTGCTGCCACCTGCT13500.10067714703338006No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12100.078.5492551
TCAACGC15250.062.3243264
ATCAACG15400.061.2497183
TATCAAC16050.058.320582
CAACGCA16550.057.428765
AACGCAG17300.055.7590076
CGCAGAG19250.049.3627748
GCAGAGT21550.044.7624559
CAGCGCT10300.043.3322079
TACATGG29700.039.2746282
GTACATG29900.039.2527351
ACATGGG29450.038.6300543
TATTGAG6550.035.1772775
GTATGTA22500.034.881691
TATGTAC22300.033.2572172
GTACACG22350.032.538495
CATGGGG19700.032.1639944
ATGGGGA9750.031.0168885
TACACGG22800.030.9419426
ACACGGG23700.030.0706797
Produced by FastQC (version 0.11.5)