FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279871

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279871
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838884
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCAACTGTCAGGCTGTTCATTTGCAGAAACA56580.6744675068305034No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG37100.44225423300480166No Hit
GTATATTACTGTGCGAGAGAAATCTTGCACTACGGGATGGACGTCTGGGG34540.4117374988675431No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG33680.40148578349330777No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG28110.3350880455462257No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT27510.32793568598280576No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT24190.28835929639854857No Hit
ATATACAGCCGTGTCCTCAACTGTCAGGCTGTTCATTTGCAGAAACAGCG23320.2779883750315896No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT22930.2733393413153666No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG21240.253193528545067No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC20340.24246498919993706No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT17610.20992175318637618No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCG17350.20682239737556088No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT17250.2056303374483242No Hit
TACCTATTCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGT17020.20288859961567987No Hit
GCTGTATATTACTGTGCGAGAGAAATCTTGCACTACGGGATGGACGTCTG17020.20288859961567987No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC16830.20062368575393025No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15000.1788089890854993No Hit
TTATTACTGCATGCAAGCTCTACAATTTCCGTGGACGTTCGGCCAAGGGA13710.16343141602414638No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATCCCGT13150.15675588043162106No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC12460.14853066693368808No Hit
CAGTAATATACAGCCGTGTCCTCAACTGTCAGGCTGTTCATTTGCAGAAA12430.14817304895551708No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12370.1474578129991751No Hit
CCTTGTAGGTCGTGGGCCCAGACTCTTTGGCCTCAGCCTGCACCTGGTCC12080.14400083921018877No Hit
ATTTATTACTGCATGCAAGCTCTACAATTTCCGTGGACGTTCGGCCAAGG12040.14352401523929412No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11970.14268957329022844No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG11950.1424511613047811No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG11940.14233195531205745No Hit
GGTATGTGCAGAAGTCAGGGCAGTCTCCACAGCTCCTGATCTCTGGGGGC11580.13804053957400544No Hit
ATTCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG11490.13696768563949246No Hit
GACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCA11330.1350603897559138No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC11300.1347027717777428No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG11190.13339150585778248No Hit
ATATTACTGTGCGAGAGAAATCTTGCACTACGGGATGGACGTCTGGGGCC11050.13172262195965115No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACT10960.13064976802513817No Hit
CAGTAATAAATACCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGT10900.12993453206879615No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC10680.1273120002288755No Hit
CCTACAAGGTGACCAGCACACTGACCATCAAAGAGAGCGACTGGCTCAGC10490.12504708636712586No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC10170.12123249459996853No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10050.11980202268728453No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT9960.11872916875277154No Hit
CTCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAACCTCCTG9960.11872916875277154No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT9720.11586822492740355No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG9660.11515298897106156No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA9300.11086157323300957No Hit
CACATGGAGGACGCATTCTGCTGGAAGGTCAGGCCCCTGTGATCCACGCG9260.1103847492621149No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCT9260.1103847492621149No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAATACCTATTCTATGCACTGGG9110.10859665937125991No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9070.10811983540036525No Hit
GTAATAAATACCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTAA9060.10800062940764159No Hit
GGGCTGGAGTGGGTGGCAGCTCTATCAAATGATGGAACTAAGACATATTA9050.10788142341491792No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8920.10633174550951024No Hit
ATATTACGCAGACTCCGTGAAGGGCCGATTCCCCATCTCCAGAGACAATT8920.10633174550951024No Hit
GAATAGGTATTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA8920.10633174550951024No Hit
CACGTGCACCGTGACCCACACAGACCTGCCCTCGCCACTGAAGCAGACCA8860.10561650955316826No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA8840.10537809756772092No Hit
ACACAGACCTGCCCTCGCCACTGAAGCAGACCATCTCCCGGCCCAAGGGG8820.10513968558227359No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT8730.10406683164776058No Hit
GTGGATGCAGAGGGGGCAGCCCTTGTCCCCGGAGAAGTATGTGACCAGCG8670.1033515956914186No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT8560.10204032977145826No Hit
GTGCTGGACTTTGCACACCACGTGTTCGTCTGTGCCCTGCATGACGTCCT8430.1004906518660506No Hit
TCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC8410.10025223988060328No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGAC300.001990101371.9945763
GGTATCA6900.063.8071171
GTATCAA16500.062.1144681
GTGCTAG3550.056.9269641
ATCAACG18050.055.840673
TCAACGC18250.055.2287184
TATCAAC18850.053.133172
CAACGCA19000.053.0486345
AACGCAG19400.051.583746
CTAGAGA4400.047.450974
GCTAGAG3900.046.1503643
GAATACG3800.045.4702579
CGCAGAG22400.044.996618
ATACCGT657.5930735E-444.3043566
CCCGTTG5300.043.4684268
CCGTTGA5300.042.110049
GTCATAG12600.041.2430081
GCAGAGT24500.041.139769
TACCGTC700.001093537941.139767
ATAGGTG12100.040.4597634
Produced by FastQC (version 0.11.5)