FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences838884
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACAGCCGTGTCCTCAACTGTCAGGCTGTTCATTTGCAGAAACA47360.5645595815392831No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG34610.41257194081660875No Hit
GTATATTACTGTGCGAGAGAAATCTTGCACTACGGGATGGACGTCTGGGG31790.3789558508685349No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28830.3436708770223297No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG25170.30004148368546785No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT20660.24627958096709437No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT19580.23340533375293843No Hit
ATATACAGCCGTGTCCTCAACTGTCAGGCTGTTCATTTGCAGAAACAGCG19020.2267297981604131No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18750.22351123635687412No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC17770.21182904906995484No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT16360.1950210040959179No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG14320.17070298158029No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC14290.17034536360211902No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13530.1612857081551204No Hit
TACCTATTCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGT13330.15890158830064705No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAACTGGTGGAGTCTGGGGGAGGCG12860.15329890664263474No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT12660.15091478678816142No Hit
GCTGTATATTACTGTGCGAGAGAAATCTTGCACTACGGGATGGACGTCTG12510.14912669689730643No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12280.1463849590646621No Hit
TTATTACTGCATGCAAGCTCTACAATTTCCGTGGACGTTCGGCCAAGGGA11200.13351071185050614No Hit
GTGTGGGTCACGGTGCACGTGAACCTCTCCCCGGAATTCCAGTCATCCTC10890.1298153260760725No Hit
CCCTGAGGAGACGGTGACCGTGGTCCCTTGGCCCCAGACGTCCATCCCGT10750.12814644217794116No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC10010.11932519871638989No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9900.11801393279642955No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCC9820.11706028485464022No Hit
GTCCAGGAGATGGTCACGCTGTCATAGGTGGTCAGGTCTGTGACCAGGCA9720.11586822492740355No Hit
ATTCTATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG9690.11551060694923254No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC9690.11551060694923254No Hit
ATTTATTACTGCATGCAAGCTCTACAATTTCCGTGGACGTTCGGCCAAGG9680.11539140095650888No Hit
CAGTAATATACAGCCGTGTCCTCAACTGTCAGGCTGTTCATTTGCAGAAA9660.11515298897106156No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9530.11360331106565388No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG9280.11062316124756223No Hit
GGTATGTGCAGAAGTCAGGGCAGTCTCCACAGCTCCTGATCTCTGGGGGC9170.1093118953276019No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG9170.1093118953276019No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT8860.10561650955316826No Hit
CAGTAATAAATACCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGT8840.10537809756772092No Hit
GAATAGGTATTGAAGGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGA8760.10442444962593159No Hit
ATATTACTGTGCGAGAGAAATCTTGCACTACGGGATGGACGTCTGGGGCC8450.10072906385149795No Hit
GGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTG8450.10072906385149795No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC9550.062.564934
ATCAACG9800.062.438023
CAACGCA10450.057.865445
CTAGTAC502.0818025E-457.5898933
GTATCAA10800.057.3711431
AACGCAG10850.055.0686766
TATCAAC11600.054.6111032
CGCAGAG13350.045.295428
CAGAGCG12800.043.86739
AATATAC21700.042.4625933
TAATATA21450.042.2862852
TATACAG23450.041.1356355
GCAGAGT14800.039.884899
GTGCTAG3450.039.6783261
ATATACA24850.039.397514
CCCCGTT4050.039.1042487
ACAGCCG22550.038.94668
CAGCCGT22300.038.73769
TAGGTGG15150.038.4882935
GGTATCA4500.038.4253271
Produced by FastQC (version 0.11.5)