FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789569
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAACATGGGATGACAGCCTGAATGGTGAGGTATTCGG41290.5229435299511506No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT23320.29535100795497293No Hit
GTAATAATCAGCCCCATCCTCAGACTGGAGCCCACTGATGGCCAGGGTGG22900.2900316501787684No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC22330.2828125217682052No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG22100.27989954012885515No Hit
ATGTATTACTGTGCGAGAACGTACAACAGCAACTGGTACGAGTACCTGGA18520.23455834765549305No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC17760.2249328431080754No Hit
GTAATACATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG16940.21454743030691428No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT16050.2032754578763857No Hit
TCTTACGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15220.19276339369960066No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC14710.18630417354278092No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC13150.16654655894544998No Hit
GATTATTACTATAGATGAGGAGTTGGGGGGCCGTTCCCGGGAGCTGCTGG13100.1659133020673304No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC12170.15413472413430618No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT12000.1519816507486996No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11720.14843541223122994No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA11680.1479288067287343No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11620.1471688984749908No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10960.13880990768381232No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10920.13830330218131665No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10820.13703678842507747No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10580.13399715541010349No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT10470.1326039902782404No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10430.13209738477574473No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG10400.131717430648873No Hit
GACGTAAGACGGTCAACTTGGTCCCTCCGCCGAATACCTCACCATTCAGG10060.12741128387765982No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10030.1270313297507881No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9910.1255115132433011No Hit
GTATTACTGTGCGAGAACGTACAACAGCAACTGGTACGAGTACCTGGACG9630.12196527472583144No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9390.11892564171085743No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9260.11727917382774652No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9200.11651926557400304No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA9070.11487279769089212No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCT8910.11284637568090947No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8830.11183316467591814No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG8750.1108199536709268No Hit
CATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG8490.10752701790470497No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG8340.10562724727034624No Hit
GCCTTGGGCTGACGTAAGACGGTCAACTTGGTCCCTCCGCCGAATACCTC8120.10284091700662006No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)