FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1153935
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC44530.3858969526013164No Hit
GTAATATTCGGCCTCATCCTCGGACCGGAGCCCACCGATGGCCAGGGAGG41150.35660587468098287No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGACCACTC38380.332601056385325No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT35110.30426323839731007No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC34890.302356718532673No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA31500.27297898061849235No Hit
CCCAAAGACCACTCAGGCTGTCATCCCATGCTGCACAGTAATATTCGGCC30520.2644863012214726No Hit
GAATATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCTTTGGGTGTT28200.24438118264893602No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT28060.24316794273507605No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC27170.23545520328268058No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA26850.23268208347957206No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC25160.21803654451940535No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24760.2145701447655197No Hit
CTATAGTAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCT24270.21032380506700982No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG24190.20963052511623273No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT23550.2040842855100157No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA22440.19446502619298314No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC22430.194378366199136No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA22380.19394506622990032No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATGATCGGCGGCCCT21800.18891878658676614No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21680.18787886666060047No Hit
GTCTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC21490.1862323267775048No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21390.18536572683903338No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA21240.18406582693132628No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT20280.1757464675220008No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20050.17375328766351658No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAAGACCACTCA19650.17028688790963095No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT19220.16656050817420393No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT19180.16621386819881534No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC18860.16344074839570688No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18570.1609276085741398No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA17890.15503472899253426No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT17400.15078838929402436No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC16820.1457621096508902No Hit
ATCCCATGCTGCACAGTAATATTCGGCCTCATCCTCGGACCGGAGCCCAC16690.14463552973087737No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16420.1422957098970046No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG15940.13813603019234186No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG15770.13666281029694047No Hit
CATCTATAGTAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG15760.13657615030309334No Hit
GCATGGGATGACAGCCTGAGTGGTCTTTGGGTGTTCGGCGGAGGGACCAA15650.1356228903707748No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG15650.1356228903707748No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCTTTGGGTGTTCGGCG15290.13250313059227772No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC15050.13042329073994635No Hit
TTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGGTGCTGGTACCA15030.1302499707522521No Hit
TATGTATACTGGTACCAGCACCTCCCAGGAACGGCCCCCAAACTCCTCAT14690.1273035309614493No Hit
ATTTATTACTGTGCGAGACATGGGTCACCATCTGGGAACTACCACTACTA14220.12323051125063371No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC13690.11863753157673525No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA13570.11759761165056958No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG13550.1174242916628753No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG13540.11733763166902815No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG13420.11629771174286246No Hit
TACCAGCACCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAATGA13400.1161243917551682No Hit
GTAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG13320.11543111180439107No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC13290.11517113182284965No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG13020.11283131198897683No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG13000.11265799200128257No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT12990.11257133200743544No Hit
GTATACTGGTACCAGCACCTCCCAGGAACGGCCCCCAAACTCCTCATCTA12940.11213803203819973No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA12880.11161807207511688No Hit
ATATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTCTTTGGGTGTTCG12630.10945157222893838No Hit
GCACAGTAATATTCGGCCTCATCCTCGGACCGGAGCCCACCGATGGCCAG12060.10451195257965137No Hit
ATGTATACTGGTACCAGCACCTCCCAGGAACGGCCCCCAAACTCCTCATC11980.10381867262887425No Hit
ATAGTAATGATCGGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCC11950.10355869264733282No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA11900.10312539267809712No Hit
GTAATAAATGGCGGTGTCCGAGGCCTTCAGGCTGCTCCACTGCAGGTAGG11870.10286541269655569No Hit
TCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCA11870.10286541269655569No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17000.068.661651
GGTATCA7650.062.717531
ATCAACG20300.056.049923
TCAACGC20700.054.5808684
CAACGCA21850.051.708195
AACGCAG23550.048.2624446
CGCAGAG26300.042.944198
ACGCAGA28400.040.0204437
TATCAAC29800.038.6652872
GCAGAGT31250.036.3595479
TATTGAG17900.034.755215
GTACATG27750.032.773011
TTATTGA18800.032.3306774
GGTTTAT13350.031.1115971
TACATGG28900.030.95452
ATTGAGT14100.030.418326
CAGAGTA30800.030.1622669
CTGATCG1504.854114E-528.5845499
TACGTTG1050.00824501227.2410835
GTATTAC3256.002665E-1126.440731
Produced by FastQC (version 0.11.5)