FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1000545
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT29720.2970381142277459No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25190.25176278927984247No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT20100.2008905146695051No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA17140.17130663788235412No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT16440.16431045080431164No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15850.15841366455281872No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC15060.15051796770759934No Hit
TCCCTATCTTTGGTAGAGCAATCTACGCACAGAAGTTCCAGGGCAGAGTC14830.14821922052481395No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG14620.14612036440140125No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC13940.1393240683827314No Hit
GTCTGGGGCTGCGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA13650.1364256480218281No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG13630.13622575696245545No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT13020.1301290796515899No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12820.12813016905786348No Hit
GATCTATGCTTCATCCCGTTTGCAAACTGGGGTCCCATCAAGGTTCAGCG12280.12273311045480215No Hit
CACCATCACCAGTCTCCAACCTGAAGATTTTGCAGCTTACTACTGTCAAC12280.12273311045480215No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG12230.12223338280637053No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT12100.12093409092044835No Hit
CCTCCATGCCGTCCACGTACCAGTTGAACTGGACCTCGGGGTCTTCGTGG12060.12053430880170307No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12000.11993463562358514No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG11900.11893518032672194No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT11810.11803567055954506No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGCGACAGAGTCACCATCACTT11540.11533714125801439No Hit
ACTGTCAACAGAGTCACAGAACCCCTCGGACGTTCGGCCAAGGGACCAGG10560.10554247934875492No Hit
GCATGGAGGTGCATAATGCCAAGACAAAGCCACGGGAGGAGCAGTTCAAC10530.10524264275969597No Hit
CATTAGCAGTGACTTAAATTGGTATCAGCAGAAGCCAGGGAAAGCCCCTA10470.10464296958157804No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC10410.1040432964034601No Hit
TTATTGCACCATTCAGTCGCGTGTGGAAGCGCGTGCCGACTACTGGGGCC10210.10204438580973368No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA10090.10084503945349783No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)