FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences922758
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC30760.3333485052418944No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGG28150.30506373285303406No Hit
GTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCCCCCTCAGCGTCT27300.2958522169409531No Hit
GTCCTGGGCCCAGTCTGCGCTGACTCAGCCCCCCTCAGCGTCTGCGACCC25290.2740696910782675No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTCAGG23410.2536959852962532No Hit
GATTATTACTGTTCAGCATGGGATGACAGCCTGAATGGTCCGGTGTTCGG23090.25022812048229326No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCCC22920.2483858172998771No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT19220.20828863038846587No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA18790.2036286870447073No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG18030.19539250811155254No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC17900.19398368803088134No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA16830.18238801505920293No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACGCAGGACAATC16320.17686110551195436No Hit
ATATATTACTGTGCGCGAGGAGGTGATGACTACAGCCTTGACTACTGGGG16300.17664436396108188No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC16250.17610251008390065No Hit
CTTATACTGTGAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTC16120.17469369000322946No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT15810.17133419596470578No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14570.15789621981061125No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14420.15627065817906755No Hit
GAACTTATACTGTGAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAA14330.1552953212001413No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG14240.1543199842212151No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14030.15204419793705393No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA13970.15139397328443643No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13760.14911818700027527No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC13220.14326616512671794No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG13090.14185734504604675No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13060.141532232719738No Hit
ATATTACTGTGCGCGAGGAGGTGATGACTACAGCCTTGACTACTGGGGCC12840.13914807566014056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12780.1384978510075231No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12600.13654717704967068No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT12580.13633043549879817No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGACCATTCAGGC12540.13589695239705318No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTG12400.13437976154094572No Hit
GGGTAGAGAAGACAGGACGCAGGACAATCTCCAGCATGGCCAGCTTCCCT12260.13286257068483828No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA12260.13286257068483828No Hit
GTAATATATGGCCGTGTCGTCAGATCTCAGGCTCCCCAGCTCCATGTAGG11970.12971981819718711No Hit
GGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCGTCTATGGTCAT11970.12971981819718711No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGG11920.1291779643200059No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG11910.12906959354456965No Hit
GTCATGATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG11890.12885285199369714No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC11410.12365105477275733No Hit
CCCAGGAACGGCCCCCAAACTCCTCGTCTATGGTCATGATCAGCGGCCCT10830.11736554979745503No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT10450.11324746033087765No Hit
TCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGCGCTGACTCAGCCC10080.10923774163973653No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC10060.10902100008886403No Hit
AACTTATACTGTGAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAAC10030.1086958877625553No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9890.10717869690644785No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC9800.10620335992752163No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG9470.10262712433812549No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG9230.10002622572765557No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19050.073.471311
GGTATCA6600.069.9595641
TCAACGC20350.067.596744
ATCAACG20550.066.5883943
CAACGCA21350.064.7644355
AACGCAG22000.063.46096
TATCAAC21900.062.4836352
CGCAGAG26800.052.0919468
ACGCAGA27750.050.0492977
TATATCG750.001532886338.408915
GCAGAGT39450.035.7530179
TTATTGA14400.035.0100174
TATTGAG14100.034.7314575
ACATGGG25650.033.4130673
GTACATG26350.032.8557741
GGTTTAT11800.030.570261
TCCGACC1453.672085E-529.7774098
CATGGGG13700.028.9133824
TTCCGAC1504.6274716E-528.7848287
ATGGGAG13150.027.9304335
Produced by FastQC (version 0.11.5)