FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736827
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACATGGAGCTGAGCAGCCTGACATCTGAGGACACGGCCGTGTATTA30180.40959411096498904No Hit
GTAATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTAGA18740.25433378527116945No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA17020.23099044958993087No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15700.2130757966252594No Hit
GTGTATTACTGTGCGAGACATAGGGTAGCAGCAGCTGGTAGCGACTACTA15220.20656137736537886No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14210.19285395350604687No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14060.19081819748733422No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13510.18335375875205442No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA12970.1760250370846888No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12950.17575360294886044No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT12370.16788201300983813No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12160.1650319545836404No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTAGCTG11980.16258904736118518No Hit
CATGTAGACTGTGCTCGTGGATTCGTCCGCGGCAATCGTGACTCTGCCCT11660.15824610118793148No Hit
CCATGTAGACTGTGCTCGTGGATTCGTCCGCGGCAATCGTGACTCTGCCC11010.14942449177350994No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTA10490.1423672042419727No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG10090.13693852152540556No Hit
TCCCTATCTTTTATACACCAAACTACGCACAGAAGTTCCAGGGCAGAGTC9880.1340884630992078No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9870.13395274603129365No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA9740.13218842414840934No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9700.1316455558767526No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9540.12947408279012576No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT8890.1206524733757042No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG8740.11861671735699153No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8460.11481663945539454No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG8200.11128799568962593No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8190.11115227862171174No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA8130.11033797621422668No Hit
CTCCTGCACTGGAACCAGCAGTGATGTAGGTGGTTATAAGTATGTCTCCT8030.1089808055350849No Hit
GTATTACTGTGCGAGACATAGGGTAGCAGCAGCTGGTAGCGACTACTACT8010.10870937139925654No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8010.10870937139925654No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7660.10395927402226031No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG7440.1009734985281484No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7430.10083778146023421No Hit
ACATGGAGCTGAGCAGCCTGACATCTGAGGACACGGCCGTGTATTACTGT7420.10070206439232006No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)