FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279884

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279884
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736827
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACATGGAGCTGAGCAGCCTGACATCTGAGGACACGGCCGTGTATTA28490.386657926487493No Hit
GTAATACACGGCCGTGTCCTCAGATGTCAGGCTGCTCAGCTCCATGTAGA17850.24225496622680762No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA16080.21823304520599815No Hit
GTGTATTACTGTGCGAGACATAGGGTAGCAGCAGCTGGTAGCGACTACTA14970.2031684506675244No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14360.19488970952475954No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13620.18484664649911037No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC13040.17697505656008805No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12840.1742607152018045No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12690.17222495918309183No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11870.16109615961412924No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTAGCTG11530.1564817793050472No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT11510.15621034516921883No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11290.15322456967510692No Hit
CATGTAGACTGTGCTCGTGGATTCGTCCGCGGCAATCGTGACTCTGCCCT10460.14196005303823014No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA10240.13897427754411823No Hit
CCATGTAGACTGTGCTCGTGGATTCGTCCGCGGCAATCGTGACTCTGCCC10100.13707423859331974No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9720.13191699001258098No Hit
TCCCTATCTTTTATACACCAAACTACGCACAGAAGTTCCAGGGCAGAGTC9460.12838834624681233No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAGCAGTGATGTA9060.12295966353024522No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8930.1211953416473609No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG8880.12051675630779003No Hit
GTATTACTGTGCGAGACATAGGGTAGCAGCAGCTGGTAGCGACTACTACT8440.1145452053195662No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA8410.11413805411582365No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8390.11386661997999531No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC8380.11373090291208113No Hit
GTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG8340.11318803464042441No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG7880.10694504951637224No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAACCAG7830.10626646417680133No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT7770.10545216176931628No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7740.10504501056557372No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT7540.10233066920729017No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7520.10205923507146182No Hit
GATTATTACTGCTGCTCATATGCAGGCAGCTACACTTGGGTGTTCGGCGG7410.10056634732440586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG8450.062.2059943
TCAACGC8600.059.4464534
CAACGCA9050.056.4905555
GTATCAA9450.056.385311
AACGCAG9700.054.9208956
GGTATCA3800.049.2669681
TGTACCG752.6551865E-548.0037085
GAATACG1551.6370905E-1146.4457479
CGCAGAG11650.045.1101848
GTGCTAG3100.037.1641651
CAGCGCT4500.036.7953539
ACGCAGA15150.034.688697
ATAATAC3750.032.642523
GCAGCGC6100.030.6846498
GGCAGCG5450.030.3814857
TCTACAT17800.030.3394242
CTACATG18150.029.7543663
GTCTACA18550.029.1127621
TAAACGC1000.006279895528.8022274
GTACATG16750.028.3723431
Produced by FastQC (version 0.11.5)