FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984029
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACATGGAGCTGACCAGCCTGACATCTGAGGACACGGCCCTGTATTA25940.26361011718150584No Hit
GTATTACTGTGCGAGCGAGCAGTGGCTGCCCACCGGACGCCTACGTCCCC17530.17814515629112557No Hit
GTAATACAGGGCCGTGTCCTCAGATGTCAGGCTGGTCAGCTCCATGTAGA16830.17103154480203325No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT16450.16716986999366887No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCTTGCA16120.16381631029166824No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14800.15040207148366563No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13980.14206898373930035No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13380.13597160246293555No Hit
CTGTATTACTGTGCGAGCGAGCAGTGGCTGCCCACCGGACGCCTACGTCC12880.1308904513992982No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12760.12967097514402523No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA12550.12753689169729754No Hit
GCCCTGTATTACTGTGCGAGCGAGCAGTGGCTGCCCACCGGACGCCTACG12250.12448820105911514No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT11900.12093139531456898No Hit
TTCCAGGACAGAGTCACAATTACCGCGGACGAATCGACGAGCACAGTCTA11590.11778108165511382No Hit
ATACAGGGCCGTGTCCTCAGATGTCAGGCTGGTCAGCTCCATGTAGACTG11460.11645998237856811No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG10500.1067041723363844No Hit
CAGTAATACAGGGCCGTGTCCTCAGATGTCAGGCTGGTCAGCTCCATGTA10260.10426521982583846No Hit
CTCTTGAACCACTCAGGCTGCTGTCGTAAGACTGGCAGTAATAATCACCT9870.10030192199620135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9350.066.237361
TCAACGC10300.059.4289254
CAACGCA10350.058.4460455
ATCAACG11050.055.3952873
AACGCAG12200.049.565696
TATCAAC12400.049.3643532
CGCAGAG14500.041.703558
GGTATCA5100.038.1249961
ACGCAGA16900.035.7811477
GCAGAGT17700.035.384069
GTACATG22350.033.8320241
TAGTACG1402.8758031E-530.863094
ACATGGG23400.029.8519063
GTAATAC13000.028.2515981
GTACACG8600.026.795865
GTGCTAG3850.026.1868651
GTCTACA17600.026.1868631
CTACATG17900.025.3456673
GTATAGT3701.0913936E-1125.3021721
TGCACCG2851.0628355E-825.2680285
Produced by FastQC (version 0.11.5)