FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739912
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAAACAGCCGTGTCGTCAGTTCTGAGGCTGTTCATTTGCAAAGACA25630.3463925439782028No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19490.2634097027754652No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG19400.26219334191092997No Hit
GTTTATTATTGTGCGACAGAGGGCCTGTGGAACGATGATGTGGTGCTGAC17730.23962309031344267No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC17040.2302976570186725No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA16800.22705402804657854No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA16330.22070192130956112No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC15620.21110618560044977No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT15270.20637589334947937No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14280.19299592383959174No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13910.18799532917428016No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13280.1794808031225335No Hit
CTCCTGTGCAGTCTCTGAATTCACCTTCACTGCTTCCTCTGGATTCACCT13240.17894019829385116No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT12650.1709662770707868No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA12380.16731719447718107No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11710.15826206359675205No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC10380.14028695304306457No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10320.13947604580004108No Hit
GGACTGGAGTGGGTGGCAGTTATTTCATATGATGGAAACAATAAATTCTA10280.13893544097135874No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG9940.13434029992755897No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT9730.13150212457697671No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9470.12798819319054158No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA9360.1265015299116652No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC9350.12636637870449458No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC9150.12366335456108293No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9060.1224469936965477No Hit
TCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC9030.12204154007503594No Hit
CTCCAGTCCCTTGCCTGGAGCCTGGCGGACCCAATGAATAGCAGAGGTTG9030.12204154007503594No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT8930.12069002800333013No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8870.11987912076030663No Hit
CTCCGTGAGGGGCCGATTCACCATCTCCGGAGACATTTCCAAGAACACGC8860.11974396955313604No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT8840.11947366713879487No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8820.11920336472445371No Hit
GTCTTTGCAAATGAACAGCCTCAGAACTGACGACACGGCTGTTTATTATT8520.11514882850933625No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC8470.11447307247348334No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8330.11258095557309519No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC8160.11028338505119528No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC8070.10906702418666003No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC8050.10879672177231886No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACACCAATCAGG8030.1085264193579777No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT8020.10839126815080712No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7760.10487733676437197No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA7740.10460703435003081No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG7680.10379612710700734No Hit
TTATTATTGTGCGACAGAGGGCCTGTGGAACGATGATGTGGTGCTGACTG7660.10352582469266615No Hit
GTTCTGAGGCTGTTCATTTGCAAAGACAGCGTGTTCTTGGAAATGTCTCC7570.10230946382813091No Hit
CCCTGAGACTCTCCTGTGCAGTCTCTGAATTCACCTTCACTGCTTCCTCT7410.1001470445134016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4350.064.219471
GTATCAA9950.063.3506281
ATCAACG11700.053.8022233
TCAACGC11700.053.769494
TATCAAC12550.050.1854062
CAACGCA12550.049.55815
AACGCAG13550.045.9006736
GAATACG1351.1636075E-842.352399
CGCAGAG14950.041.6022838
TAGTACG700.001132024240.8508454
GTACATG15550.039.6149751
ACATGGG15500.039.22753
TACATGG15950.038.141412
GCAGAGT17750.035.0301979
CATGGGA8400.033.191314
CAGAGTA17450.031.536759
CAGCCGT15600.029.7790249
GGGCAGT6350.029.3285751
GTGGTAT2354.8285074E-827.4325391
GGTGGGT6800.027.3340238
Produced by FastQC (version 0.11.5)