FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739912
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATAAACAGCCGTGTCGTCAGTTCTGAGGCTGTTCATTTGCAAAGACA24270.32801197980300356No Hit
GTTTATTATTGTGCGACAGAGGGCCTGTGGAACGATGATGTGGTGCTGAC20590.27827633556422926No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT19420.2624636443252711No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG16380.22137767734541403No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA15850.21421466336537318No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC15790.2134037561223497No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15700.2121873952578144No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC14160.19137410935354474No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13580.18353533933765095No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13410.18123776881575104No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG13050.17637232535761008No Hit
CTCCTGTGCAGTCTCTGAATTCACCTTCACTGCTTCCTCTGGATTCACCT12460.16839840413454574No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12450.16826325292737515No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12430.16799295051303398No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT12080.1632626582620636No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA11920.16110023894733427No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11150.15069359599519944No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA11130.15042329358085826No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT10820.1462336061585702No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG10380.14028695304306457No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC10040.13569181199926478No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9550.12906940284790624No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC9530.12879910043356507No Hit
GGACTGGAGTGGGTGGCAGTTATTTCATATGATGGAAACAATAAATTCTA9440.12758273956902982No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8810.11906821351728314No Hit
CTCCAGTCCCTTGCCTGGAGCCTGGCGGACCCAATGAATAGCAGAGGTTG8730.11798700385991848No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC8490.1147433748878245No Hit
GTCTTTGCAAATGAACAGCCTCAGAACTGACGACACGGCTGTTTATTATT8480.1146082236806539No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC8460.11433792126631274No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC8190.11068883867270701No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG8050.10879672177231886No Hit
CTCCGTGAGGGGCCGATTCACCATCTCCGGAGACATTTCCAAGAACACGC7970.1077155121149542No Hit
TTATTATTGTGCGACAGAGGGCCTGTGGAACGATGATGTGGTGCTGACTG7950.10744520970061304No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7940.10731005849344247No Hit
CATTTACCCGGGGACAGGGAGAGGCTCTTCTGCGTGTAGTGGTTGTGCAG7820.10568824400739547No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGGGGCCC7810.10555309280022489No Hit
TCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC7760.10487733676437197No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC7700.1040664295213485No Hit
GTTCTGAGGCTGTTCATTTGCAAAGACAGCGTGTTCTTGGAAATGTCTCC7600.10271491744964266No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC7470.10095795175642508No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT7460.10082280054925451No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT7410.1001470445134016No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)