FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences846089
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACGGCCGTGTCCTCAAATCTCAGGCTGCTCAGCTCCATGTAGG20200.23874556931953966No Hit
GTGTATTACTGTGCGAGAGGTTCAGGCGCCTATGATACTAGTGGTTATTG15460.1827230941425784No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCG14260.16854018903448692No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT13660.1614487364804412No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13060.15435728392639544No Hit
TCCCTATCTTTGGTACAACAACGTACGCACAGAACTTCCAGGGCAGAGTC11530.13627407991357882No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11380.1345012167750674No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11300.1335556897678613No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT11210.13249197188475442No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11160.13190101750525063No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10990.12989177261493767No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA10920.12906443648363233No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10070.11901821203206754No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9390.11098123247081572No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATTTGAGGACACGGCCGTGTATTA9290.1097993237118081No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9190.10861741495280046No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG9060.10708093356609055No Hit
GGCTAACACTTTCCCATTCACTTTCGGCCCTGGGACCAAAGTGGAGATCA8570.10128958064695322No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8510.10058043539154865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG15500.066.965513
TCAACGC15800.065.6940164
CAACGCA17000.061.0531775
AACGCAG18550.056.283636
CGCAGAG21300.049.0024768
GTATCAA24300.046.587351
ACGCAGA23450.044.5097127
TATCAAC27250.040.4711272
GTACACG7750.031.6207285
ACACGGG7850.031.177337
ATACACG11100.031.170134
GTACATG20550.030.5268541
CAGCGCT4750.030.308619
ACATGGG20300.029.8265913
GCAGAGT35950.029.6341489
GGTATCA12750.028.8426131
GTAATAC12800.028.7299441
GTATGTA10800.028.7090821
TAGGACG1306.705568E-427.7234344
AATACAC14150.027.5079153
Produced by FastQC (version 0.11.5)