FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160079
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACACCCGTGTCCTCGGCTCGCAGACTGTTCATTTGCAGAAACA36250.312478719121715No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28220.24325929527213233No Hit
ATATACACCCGTGTCCTCGGCTCGCAGACTGTTCATTTGCAGAAACAGGG26740.23050154342936988No Hit
GATTAAGACTGATGGGAGTGTAACAAGCTACGCGGGCGCCGTGAAGGGCC23070.19886576690035765No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT21110.1819703658112939No Hit
GTATATTATTGTGCAAGAGATCTTAGGGGATCCAACTTCTGGGGCCGGGG20280.17481568065623118No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG19940.1718848457734344No Hit
GGTGTATATTATTGTGCAAGAGATCTTAGGGGATCCAACTTCTGGGGCCG19830.17093663448782367No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG19260.16602317600784086No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT18130.1562824600738398No Hit
GTCTTAATCTGTGCGACCCACACCGGCCCCTTCCCTGGAGGTTGGCGGAT17670.1523172128794677No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG16240.1399904661665283No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC16080.13861124975109454No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15670.13507700768654549No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCT14770.1273189153497305No Hit
GTAATAATCCGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG12580.10844089066348067No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT12520.107923684507693No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACCAGCGGTGACGTT12430.10714787527401151No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG11960.10309642705367478No Hit
CAATAATATACACCCGTGTCCTCGGCTCGCAGACTGTTCATTTGCAGAAA11920.10275162294981635No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCC11710.10094140140455951No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG19800.062.1745953
TCAACGC19800.062.1745954
CAACGCA20550.059.9054455
AACGCAG22000.055.9571386
ACGCAGA23050.053.408117
CGCAGAG24350.050.5567558
GCAGAGT28650.042.968839
ATAGGTG14700.035.261214
GTCATAG15100.034.90471
TATCAAC36600.034.468292
GTACATG24100.034.4523471
TAGGTGG15450.033.5495035
TACATGG24400.032.2032282
ACATGGG26000.030.181113
CATAGGT18450.028.8746933
ATACGTA2003.461846E-728.7966566
AATATAC19300.027.603013
TCATAGG20150.027.1895052
GTGGTCA19250.026.9267438
ATACACC19550.026.8817866
Produced by FastQC (version 0.11.5)