FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279892

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279892
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160079
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACACCCGTGTCCTCGGCTCGCAGACTGTTCATTTGCAGAAACA28220.24325929527213233No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG27630.2381734347402203No Hit
ATATACACCCGTGTCCTCGGCTCGCAGACTGTTCATTTGCAGAAACAGGG20700.17843612374674483No Hit
GTATATTATTGTGCAAGAGATCTTAGGGGATCCAACTTCTGGGGCCGGGG18850.1624889339432918No Hit
GATTAAGACTGATGGGAGTGTAACAAGCTACGCGGGCGCCGTGAAGGGCC18310.1578340785412028No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGG16750.1443867184907235No Hit
GGTGTATATTATTGTGCAAGAGATCTTAGGGGATCCAACTTCTGGGGCCG16590.1430075020752897No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT16420.1415420846338913No Hit
GTCTTAATCTGTGCGACCCACACCGGCCCCTTCCCTGGAGGTTGGCGGAT16220.13981806411459907No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG15970.1376630384654838No Hit
TCTCAGGACTGATGGGAAGCCCCGGGTGCTGCTGATGTCAGAGTTGTTCT15290.13180136869989026No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGC14190.12231925584378306No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC14010.12076763737642006No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT13100.11292334401364044No Hit
GTCCTGAGAGGGGGCAAGTACGCAGCCACCTCACAGGTGCTGCTGCCTTC12700.109475302975056No Hit
CCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGTG12150.1047342465470024No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC11100.064.853914
GTATACG451.231298E-464.044411
ATCAACG11950.059.0360573
CAACGCA13100.054.403035
AACGCAG14500.049.150326
CGCAGAG14400.048.4918148
ACGCAGA14800.047.6676257
GTACATG23200.037.5777781
GCAGAGT19100.036.1823739
TACATGG24000.035.0940782
ACATGGG24600.033.0675853
TATACTG5700.031.5736165
AATATAC20350.031.4836273
ACACCCG19500.031.3793188
GTCATAG14750.030.773881
CACCCGT19450.030.7197489
ATACACC21650.030.258176
TACACCC20750.029.1420657
TAATATA22400.028.6023142
ATAGGTG15300.028.2305284
Produced by FastQC (version 0.11.5)