FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences566408
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATTACTGTGCGAAAGATTTCGGTGCCTGGTATCTCGGGGGGGTATA13140.2319882487535487No Hit
GTAATATATGGCCGTGTCGTCGGCTCTCAGGCTGTTCATTTGTAGAGACA12320.21751105210378383No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8530.1505981553932854Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT7270.12835270688267114Clontech Universal Primer Mix Long (96% over 26bp)
GCCATATATTACTGTGCGAAAGATTTCGGTGCCTGGTATCTCGGGGGGGT7210.12729339981073715No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)