FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564299
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAATCAGCCTCATCCTCGGACCGGAGCCCCCTGATGGCCAGGGAGG291041.1349690500210778No Hit
GATTACTACTGTGCAACATGGGATGACATCCTGAGTGGTCGGGTGTTCGG233220.9094883240994908No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC150170.5856181357946167No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC136800.5334791301638381No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGACCACTCAGG130300.5081310720785681No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT127780.4983038249439711No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG107940.420933752265239No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC97020.37834901468198523No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC87850.342588754275535No Hit
TATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCAT86890.3388450410814028No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT85720.33428239062605414No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT82310.3209844093843971No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA80350.3133409949463772No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCAC78790.30725746100591234No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC77880.30370873287397454No Hit
CTCCTCATCTATGCCAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATT75260.29349151561498876No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG73890.2881489249108626No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGACCACTCAGGA72870.2841712296420971No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT68310.26638859196996917No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA64860.2529346226785566No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA64270.2506337989446628No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC63790.24876194234759674No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT61320.2391296802751941No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA61210.2387007131383665No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT60610.23636089239203384No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA59280.2311742897376632No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG58290.2273135855062144No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC57700.2250127617723206No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT56440.2200991382050221No Hit
GGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATGCCAAT56180.2190852158816113No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA54250.21155879248090803No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT53700.2094139567967698No Hit
CTCAGGATGTCATCCCATGTTGCACAGTAGTAATCAGCCTCATCCTCGGA52540.20489030335386008No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA52410.20438334219215465No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG50300.1961549725675516No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATGCCAATAATCAGCGGCCCT50040.1951410502441408No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC49860.194439104020241No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA49000.19108536095049758No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC47860.1866397015324656No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG47320.18453386286076623No Hit
GTATACATAATTACCTCCGATGTTGGAGCTGCTTCCAAAACAAGAGATGG46510.1813751048532172No Hit
GATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATACAT46450.18114112277858394No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA43900.1711968846066703No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC42970.16757016244985473No Hit
GTAATAGACGGCAGTGTCCTCAGCCTTTAGGGTGGTGATCTGGAGATATG42810.1669462102508327No Hit
ATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATC42560.16597128493986074No Hit
GTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTA42090.16413842535523354No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG41920.1634754761437726No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC41310.16109665838500112No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG40540.1580938884272076No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG39430.15376522004649223No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC38130.14869560842943821No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG37980.14811065324285508No Hit
GTCTATTACTGTGCGAGAGTCGACTACGCAAAAACGATAGATGACTACTG37960.1480326592179773No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT37750.1472137219567609No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG37680.14694074286968875No Hit
TCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCA37460.14608280859603345No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG37450.14604381158359458No Hit
CATCCTGAGTGGTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAG37410.14588782353383908No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36830.1436259968123842No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACG36450.14214411033970686No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG35590.13879036726996344No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCC35530.13855638519533017No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA35270.13754246287191937No Hit
TTACTACTGTGCAACATGGGATGACATCCTGAGTGGTCGGGTGTTCGGCG35250.13746446884704164No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA34710.13535863017534228No Hit
ATGTTGCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCCCTGATG34430.1342667138270537No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC34390.1341107257772982No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG34340.13391574071510381No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGT33730.13153692295633232No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC33420.13032801557072712No Hit
GTGCAACATGGGATGACATCCTGAGTGGTCGGGTGTTCGGCGGAGGGACC32430.1264673113392783No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG31580.12315256528197376No Hit
CCAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG31550.12303557424465712No Hit
TTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCA31370.12233362802075733No Hit
CCCCTGAGGGCCGCTGATTATTGGCATAGATGAGGAGTTTGGGGGCCGTT31240.12182666685905193No Hit
ACATGGGATGACATCCTGAGTGGTCGGGTGTTCGGCGGAGGGACCAAGCT30770.1199938072744247No Hit
ATCCCATGTTGCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCCC30470.1188238969012584No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT30360.11839492976443075No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC30270.11804395665248085No Hit
CAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCCCTGATGGCCAGGGA30040.11714702536638669No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT29450.11484620163249293No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA29410.11469021358273743No Hit
CTACTGTGCAACATGGGATGACATCCTGAGTGGTCGGGTGTTCGGCGGAG28360.11059552727665534No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA28160.1098155870278778No Hit
GGTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTG28140.10973759300300004No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG26440.10310810088839094No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA26390.10291311582619656No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGT26340.10271813076400219No Hit
ATTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTC26290.10252314570180779No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC26280.1024841486893689No Hit
GTAATTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTC26180.10209417856498013No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC25760.10045630404254731No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTG25690.10018332495547516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42500.081.35731
GGTATCA15050.079.4541
ATCAACG52450.065.760453
TCAACGC52500.065.560674
TATCAAC54150.063.959412
CAACGCA55600.061.90415
AACGCAG58550.058.7828186
CGCAGAG67650.050.7681668
ACGCAGA73000.047.0474857
GCAGAGT76150.045.1013349
GAATACG5550.041.5143179
TATTGAG47900.033.6727685
GTACATG62550.033.6279031
TACATGG61200.033.0607532
ATTACTA106650.032.406952
TACTACT108750.032.24594
TTACTAC109050.031.8270323
GATTACT110600.031.3932381
CTACTGT111400.031.2830816
TACTGTG133750.031.0615377
Produced by FastQC (version 0.11.5)