FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2564299
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAATCAGCCTCATCCTCGGACCGGAGCCCCCTGATGGCCAGGGAGG267421.0428581066404503No Hit
GATTACTACTGTGCAACATGGGATGACATCCTGAGTGGTCGGGTGTTCGG212360.8281405561519931No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC132350.5161254596285378No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC119620.46648226279384736No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGACCACTCAGG115910.45201437117902393No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT112160.4373904915144451No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCG98120.3826386860502617No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC89610.3494522284647773No Hit
TATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCAT79570.3102992279761448No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC77640.3027728045754415No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT74230.2894748233337844No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT74120.2890458561969568No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA72170.28144143877137573No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC72010.28081748657235367No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACGGTCAC70800.27609884806724955No Hit
CTCCTCATCTATGCCAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATT69330.2703662872387346No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG67040.2614359713902318No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCGACCACTCAGGA64190.2503218228451518No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA60700.23671186550398376No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT59210.23090131065059105No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA58280.22727458849377552No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA55620.2169013831850342No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC55450.21623843397357329No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT55080.21479554451333482No Hit
GGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATGCCAAT51660.20145856625923886No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT51630.20134157522192225No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA51590.20118558717216675No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG51360.20028865588607256No Hit
CTCAGGATGTCATCCCATGTTGCACAGTAGTAATCAGCCTCATCCTCGGA50990.19884576642583413No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC50620.19740287696559566No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA50400.19654494269194037No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT48870.19057839978879218No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT48740.19007143862708678No Hit
GTATACATAATTACCTCCGATGTTGGAGCTGCTTCCAAAACAAGAGATGG46520.18141410186565607No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA46450.18114112277858394No Hit
GATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTATACAT45950.17919127215664007No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC45070.17575953506201888No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA44930.17521357688787462No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG44000.17158685473105906No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTATGCCAATAATCAGCGGCCCT43480.16955901008423743No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG42690.16647824610156617No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC41440.16160361954670652No Hit
GTAATAGACGGCAGTGTCCTCAGCCTTTAGGGTGGTGATCTGGAGATATG39560.15427218120819763No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA39090.15243932162357043No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG38170.14885159647919372No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC38080.14850062336724382No Hit
ATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATC36990.14424994901140623No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG36980.14421095199896736No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG36650.14292405058848442No Hit
GTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTA36500.14233909540190126No Hit
TCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCA36260.1414031671033682No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC36230.14128617606605157No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG36170.14105219399141833No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT35520.1385173881828913No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAG35160.13711349573509174No Hit
GTCTATTACTGTGCGAGAGTCGACTACGCAAAAACGATAGATGACTACTG34770.1355926122499755No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC33970.13247285125486538No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33670.13130294088169905No Hit
CATCCTGAGTGGTCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAG33640.13118594984438242No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG33520.1307179856951159No Hit
CCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCTGTAGGGGCCA32770.12779320976220013No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGACG32220.12564837407806187No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGG32140.12533639797855087No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC31610.12326955631929037No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGT31570.12311356826953487No Hit
TTACTACTGTGCAACATGGGATGACATCCTGAGTGGTCGGGTGTTCGGCG31450.12264560412026836No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC31440.12260660710782947No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG31270.12194365789636856No Hit
ATGTTGCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCCCTGATG31250.12186566387149081No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCC30780.12003280428686358No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG30060.11722501939126442No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA29890.11656207017980352No Hit
TTATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCA29880.11652307316736465No Hit
CCCCTGAGGGCCGCTGATTATTGGCATAGATGAGGAGTTTGGGGGCCGTT29660.11566513889370936No Hit
GTGCAACATGGGATGACATCCTGAGTGGTCGGGTGTTCGGCGGAGGGACC29570.11531416578175946No Hit
ATCCCATGTTGCACAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCCC28690.11188242868713828No Hit
CCAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG28410.11079051233884973No Hit
GCTCTAGTCTCCCGTGGGGGGGGTGAGGGTTTAGAACCTATGAACATTCT28120.10965959897812227No Hit
ACATGGGATGACATCCTGAGTGGTCGGGTGTTCGGCGGAGGGACCAAGCT27820.10848968860495597No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGC27430.10696880511983978No Hit
CTACTGTGCAACATGGGATGACATCCTGAGTGGTCGGGTGTTCGGCGGAG27130.10579889474667345No Hit
CAGTAGTAATCAGCCTCATCCTCGGACCGGAGCCCCCTGATGGCCAGGGA26760.104356005286435No Hit
GGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGT26330.10267913375156329No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT25970.10127524130376371No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16800.090.856841
GTATCAA45850.076.318171
ATCAACG52250.065.59983
TCAACGC52600.065.02644
TATCAAC55100.062.3373952
CAACGCA59050.057.4358185
AACGCAG60650.056.0415276
CGCAGAG65500.051.672018
ACGCAGA71700.047.304297
GCAGAGT73750.045.5890439
GAATACG4500.036.797729
TACATGG56850.035.9724272
GTACATG57850.035.7198451
TATTGAG45350.033.9796075
GACGGCA19400.033.778357
AGACGGC19650.033.34866
CGGCAGT19750.033.172639
ATTACTA105350.032.3302042
TTACTAC108700.031.9962773
TACTACT109350.031.8060844
Produced by FastQC (version 0.11.5)