FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005279901

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279901
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1967406
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCC59040.3000905761190115No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT54300.27599793840213965No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC50650.25744559079315604No Hit
ACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCTTTGACAC46900.23838495968803594No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCT45970.23365792317396614No Hit
GACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAAACCTTTGACA45530.23142147579096536No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA44810.2277618346187823No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACA43180.21947681363175672No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA38300.19467257902029372No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC35730.18160969316958472No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT35420.18003401433156144No Hit
GCATGGGATGACCGTGTCAAAGGTTTTGTCTTCGGAACTGGGACCACGGT34770.17673017160667398No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTC33970.17266390363758166No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC33540.17047828460419456No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33180.16864846401810302No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCG32510.16524296459398824No Hit
GTAAAAATCAGCCTCATCCTCAGACTGGAGCCCACTGATGGCCAGGGAGG32050.16290486051176015No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT30710.15609386166353056No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG30600.15553474981778037No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA30450.15477232457357554No Hit
TCCTCAGACTGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTT29960.15228173544250653No Hit
ATCCCATGCTGCACAGTAAAAATCAGCCTCATCCTCAGACTGGAGCCCAC29500.14994363136027847No Hit
ACTGTAAACTGGTACCAGCAACTCCCAGGAGCGGCCCCCAAACTCCTCAT28710.1459281917407998No Hit
GAGTAGCACTTAGTGCCAGTGCAATGTTGGCCCTCCACGCCATTCGCGCA28580.1452674231958223No Hit
GTGCAGCATGGGATGACCGTGTCAAAGGTTTTGTCTTCGGAACTGGGACC26680.13561003676922812No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26520.13479678317540963No Hit
GATTTTTACTGTGCAGCATGGGATGACCGTGTCAAAGGTTTTGTCTTCGG26060.13245867909318157No Hit
GTCTGAGGATGAGGCTGATTTTTACTGTGCAGCATGGGATGACCGTGTCA25860.1314421121009085No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT25590.13006974666133986No Hit
GTACATGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATC24920.12666424723722505No Hit
ATATTATGTAGACTCCGTGAAGGGCCGATTCACCCTCTCCAGAGACGATT24570.12488525500074718No Hit
GTGCTACTCTGGTCCATTCTACTACTACGGTATGCATGTCTGGGGCCAAG23480.11934496489285894No Hit
GCCTTGGGCTGACCTAGGACGGTGACCGTGGTCCCAGTTCCGAAGACAAA23460.11924330819363162No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTCCTGACGCAGCCACCCTCAGCGTCT23190.11787094275406296No Hit
TTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACAC23020.11700686081063086No Hit
ATGCTGCACAGTAAAAATCAGCCTCATCCTCAGACTGGAGCCCACTGATG22240.11304224954076586No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC21720.11039917536085588No Hit
TTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGG21640.10999254856394665No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG21590.10973840681587836No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCGGCTGGTGGAGTCTGGGGGAGGCG21590.10973840681587836No Hit
CTCCAGAGACGATTCCAAGAACACCGTCTATCTCCAAATGAACAGCCTGA21010.10679036253828644No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTCCTGACGCAGCCAC20830.1058754522452407No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG20430.10384231826069454No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGG20390.10363900486223991No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG20080.10206332602421665No Hit
GTCCTGGGCCCAGTCTGTCCTGACGCAGCCACCCTCAGCGTCTGGGACCC20070.102012497674603No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)