FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160527
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG114380.5294078713202843No Hit
GATGTATACTGGTACCAGCAGTTCCCAGGAACGGCCCCCACACTCCTCAT96690.4475296999296931No Hit
GACTATTACTGTGCAGCATACGATGACGCTTTGGGTGGCCCGCTGTTCGG91120.42174895291750575No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC87760.40619719170369084No Hit
GTAATACATGGCCGTGTCCTCGACTCTCAGGCTACTCATTTGTAGAAACA80890.37439939422187274No Hit
GTCCTGGGCCCAGTCTGTGCTGAATCAGCCACCCTCAGCGTCTGGGACCC77950.36079160315978465No Hit
GACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAGCGGGCCACCCAAA74170.3432958717942428No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGAATCAGCCACCCTCAGCGTCT72890.3373713913318371No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAGCGG65290.3021947885863032No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC61030.2824773770473593No Hit
ATCGTATGCTGCACAGTAATAGTCAGCCTCATCCTCGGACCGGAGCCCAC57450.2659073457540683No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGAATCAGCCAC57020.2639170905987289No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT53710.24859675440297668No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT53210.24628250422234949No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA51300.23744206853235345No Hit
ATGTATTACTGTGCGAGAGGAGATGGAGGTGAGGCGCAGTGGCCGGCCGC50630.23434097329031295No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA50050.2316564430807854No Hit
GTGTGAGGTGCAGCTGGTGGAGTCTGGGGGAGGCCTGGTACAGCCCGGGG48530.22462112253167862No Hit
GCATACGATGACGCTTTGGGTGGCCCGCTGTTCGGCGGAGGGACCAGGCT47490.21980748215597398No Hit
GTATACATCATTAGTTCCGATGTTGGAGCTGCTTCCAGAACAAGACATGG47100.2180023670150847No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA47040.21772465699340945No Hit
ACCTAGGACGGTCAGCCTGGTCCCTCCGCCGAACAGCGGGCCACCCAAAG43360.20069177566399307No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGCCTCCAAG42890.19851638049420348No Hit
GTGCTGAATCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC42580.1970815453822146No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT40110.18564914948991612No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39650.18352003932373906No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC38800.17958581401667278No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT38400.177734413872171No Hit
GATTATTCCTATCGATGAGGAGTGTGGGGGCCGTTCCTGGGAACTGCTGG38310.1773178488396581No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT38230.17694756881075774No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA38060.1761607237493445No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37270.17250420846395348No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA36980.17116194335918966No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA36970.17111565835557713No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC36810.17037509829777642No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG36790.17028252829055132No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36270.16787570810269903No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA35670.16509860788594632No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC34250.15852613737296503No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG33570.155378757127312No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG32950.15250908690333423No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG32630.1510279667877328No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCC32460.15024112172631954No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT32410.15000969670825684No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT32360.14977827169019411No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCC31680.14663089144454108No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA31600.14626061141564073No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA31330.145010916318102No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA31240.14459435128558912No Hit
GCCCCCACACTCCTCATCGATAGGAATAATCAGCGGCCCTCAGGGGTCCC30940.1432058011772128No Hit
GTATACTGGTACCAGCAGTTCCCAGGAACGGCCCCCACACTCCTCATCGA30910.14306694616637516No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30900.1430206611627626No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG30740.1422801011049619No Hit
CTATTACTGTGCAGCATACGATGACGCTTTGGGTGGCCCGCTGTTCGGCG30360.1405212709676852No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC30330.14038241595684758No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT30290.1401972759423974No Hit
ATACTGGTACCAGCAGTTCCCAGGAACGGCCCCCACACTCCTCATCGATA28810.1333470954077408No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC28270.1308477052126634No Hit
CAGTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGA28200.13052371018737557No Hit
GTATTACTGTGCGAGAGGAGATGGAGGTGAGGCGCAGTGGCCGGCCGCTC28140.13024600016570032No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA27350.1265894848803093No Hit
TTACTGTGCAGCATACGATGACGCTTTGGGTGGCCCGCTGTTCGGCGGAG27040.12515464976832041No Hit
GTGTAGGCTCTGGATTAAACCTCAGAACCTATGCCATGAGCTGGGTCCGC26950.12473808473580751No Hit
CTCCTCATCGATAGGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATT26810.12409009468523188No Hit
CAGTAATACATGGCCGTGTCCTCGACTCTCAGGCTACTCATTTGTAGAAA26730.12371981465633154No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGA26610.12316439461298101No Hit
TCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGG26090.12075757442512869No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25910.11992444436010288No Hit
ATACATGGCCGTGTCCTCGACTCTCAGGCTACTCATTTGTAGAAACAGAG25800.11941530932036488No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT25720.11904502929146454No Hit
GGTACCAGCAGTTCCCAGGAACGGCCCCCACACTCCTCATCGATAGGAAT25550.11825818423005129No Hit
TCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGAATCAGCCA25120.11626792907471187No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG24990.11566622402774879No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT24580.11376853887963445No Hit
GAACTAATGATGTATACTGGTACCAGCAGTTCCCAGGAACGGCCCCCACA24580.11376853887963445No Hit
GGCCCAGTCTGTGCTGAATCAGCCACCCTCAGCGTCTGGGACCCCCGGGC24450.11316683383267138No Hit
GTGCATACCACGCAGACTCCGTGAGGGGCCGGTTCACCATCTCCAGAGAC24140.1117319987206825No Hit
GCACAGTAATAGTCAGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAG23830.11029716360869363No Hit
GTGTGGGGGCCGTTCCTGGGAACTGCTGGTACCAGTATACATCATTAGTT23630.10937146353644273No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC23510.1088160434930922No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG23160.10719606836665314No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTG23140.10710349835942805No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTG22890.10594637326911444No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG22730.10520581321131373No Hit
GTCTTGTTCTGGAAGCAGCTCCAACATCGGAACTAATGATGTATACTGGT22700.1050669582004761No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT22510.10418754313183773No Hit
CTCCTGTGTAGGCTCTGGATTAAACCTCAGAACCTATGCCATGAGCTGGG22510.10418754313183773No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGAATCAGCCACCCTCAGCGT22460.10395611811377503No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT22040.10201214796204815No Hit
GGACTAAACTGAGAGAACTCACCATGGAGATAGGGCTGAGCTGGCTTGTT21770.10076245286450944No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT21650.1002070328211589No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA21640.10016074781754637No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13200.099.262881
GTATCAA41350.081.655751
TATCAAC50150.067.1962052
ATCAACG51400.065.562063
TCAACGC52600.064.0663454
CAACGCA58200.058.1493345
AACGCAG60100.056.31236
CGCAGAG64200.052.379558
GCAGAGT70450.047.6205449
GAATACG5950.041.138789
TATTGAG37750.037.7674875
GTGCTAG7550.037.1883661
CAGCGCT11350.036.7893039
TTATTGA40050.035.4187774
ATACCGT1854.474714E-935.030856
ATTGAGG17950.034.0984276
CTAGAGA8450.034.085774
GTAATAC41750.032.9356541
GTACATG56500.032.8746151
ACATGGG56850.032.2982943
Produced by FastQC (version 0.11.5)