FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005279903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005279903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1515467
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCGTCAGATCTCAGGCTGCTCAGCTCCATGTAGG65620.43300184035680095No Hit
GTAATAATCAGCCTCATCCTCGGACCGGAGTCCACTGATGGCCAGGGAGG58890.38859308714739416No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAGTGGTGTGCTATTCGG48550.3203632939549327No Hit
GTCTATTACTGTGCGAGCCCTACCCATTGTAGTAGTTCCTACTGCTATAG42300.2791218812418878No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC39420.2601178382637167No Hit
GCTATAGATGGCGCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTC37300.24612875107145193No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGACGACACGGCCGTCTATTA35100.23161177379646009No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA33610.2217798210056702No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT32990.21768867286453614No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC32880.21696282400078654No Hit
ATCTATAGCAGTAGGAACTACTACAATGGGTAGGGCTCGCACAGTAATAG32030.21135399187181245No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC31770.20963834910294976No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA29610.19538531686932145No Hit
TCTGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCAT29380.19386763288148143No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC28230.18627921294228114No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCACACCACTCAGG27520.18159418845807926No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC27390.1807363670736479No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT26940.1777669853583087No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT26810.17690916397387738No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT26810.17690916397387738No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA26480.1747316173826286No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC25260.16668129362104223No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC24730.16318402182297603No Hit
GGAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG24640.1625901454799082No Hit
GTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTA23680.15625546448718447No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG23300.15374798659423136No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23120.15256023390809564No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG23040.15203234382536868No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA22900.1511085361805965No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA22190.1464235116963946No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCAC21560.14226637729491964No Hit
CCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAAATGTGACTCTGCCC21310.14061672078639784No Hit
TTGTAGTAGTTCCTACTGCTATAGATGGCGCTTTGACTACTGGGGCCAGG21180.1397588994019665No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21140.13949495436060302No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT21010.1386371329761717No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT20820.13738339402969513No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG20810.13731740776935425No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC20680.13645958638492292No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA20320.13408408101265154No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20180.13316027336787933No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA19760.13038885043356274No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC19730.1301908916525401No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19360.12774940001992785No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG19010.12543988090799735No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA18710.12346029309777118No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC18410.12148070528754504No Hit
CATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAAATGTGACTCTGCCCT18280.12062288390311368No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT18270.12055689764277282No Hit
CTATAGCAGTAGGAACTACTACAATGGGTAGGGCTCGCACAGTAATAGAC18110.11950111747731887No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT17480.11534398307584395No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT17430.11501405177413958No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT17360.11455214795175349No Hit
GGGTAGGGCTCGCACAGTAATAGACGGCCGTGTCGTCAGATCTCAGGCTG17280.11402425786902651No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA17160.11323242274493604No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA17150.11316643648459518No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA16920.11164875249675511No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAGCTACAGG16670.10999909598823332No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC16650.10986712346755159No Hit
CTATTACTGTGCGAGCCCTACCCATTGTAGTAGTTCCTACTGCTATAGAT16330.10775556313664368No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16260.10729365931425759No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG16260.10729365931425759No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG16200.10689774175221235No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA16130.10643583792982625No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG15950.10524808524369056No Hit
GGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGGAAT15870.10472019516096359No Hit
GTCCCAGGTGCACCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGT15860.10465420890062273No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG15840.10452223637994096No Hit
AGCTACAGGTGTCCAGTCCCAGGTGCACCTGGTGCAGTCTGGGGCTGAGG15700.10359842873516875No Hit
CTGCTATAGATGGCGCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCG15400.10161884092494261No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC15290.10089299206119302No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG15280.10082700580085215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.077.508671
GTATCAA28600.068.847661
TCAACGC30250.064.2924
ATCAACG30350.064.080173
CAACGCA31350.062.4915475
AACGCAG31700.061.7567146
TATCAAC32550.059.74912
CGCAGAG35600.054.3847128
ACGCAGA43400.044.776347
CAGCGCT7400.042.7952429
TATTGAG20250.036.9911845
GTACATG40000.036.9646341
ACATGGG40700.036.1039163
GGCAGCG9150.034.6103637
GTACACG16000.032.8619085
TTATTGA22250.032.697154
GCAGCGC9150.032.2505658
TAGACGG35300.031.6261925
GTATGTA18350.031.444631
ATAGACG36100.031.3264394
Produced by FastQC (version 0.11.5)